[1]邵倩,班博,施锦绣.SUR1和KCNJ11基因多态性与磺脲类药物继发性失效的相关性研究[J].中国药理学通报,2013,(07):927-930.
 SHAO Qian,BAN Bo,SHI Jin xiu.Relations of SUR1 and KCNJ11 gene polymorphisms with secondary failure of sulfonylurea in type 2 diabetes[J].Chinese Pharmacological Bulletin,2013,(07):927-930.
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SUR1和KCNJ11基因多态性与磺脲类药物继发性失效的相关性研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2013年07期
页码:
927-930
栏目:
论著
出版日期:
2013-07-09

文章信息/Info

Title:
Relations of SUR1 and KCNJ11 gene polymorphisms with secondary failure of sulfonylurea in type 2 diabetes
作者:
邵倩12班博2施锦绣3
1.山东大学医学院,山东 济南250012;2.济宁医学院附属医院内分泌科,山东 济宁272029; 3.上海人类基因组研究中心,上海201203
Author(s):
SHAO Qian12 BAN Bo2 SHI Jinxiu3
1.Shandong University School of Medicine, Jinan250012,China; 2.Dept of Endocrinology, Affiliated Hospital of Jining Medical College, Jining Shandong272029,China; 3. Shanghai Human Genome Center, Shanghai201203,China
关键词:
2型糖尿病磺脲类药物基因多态性SNaPshot技术SUR1KCNJ11
Keywords:
type 2 diabetes mellitus sulfonylurea gene polymorphism SNaPshot SUR1 KCNJ11
分类号:
R394.2;R587.102.6;R977.15
文献标志码:
A
摘要:
目的探讨磺脲类药物受体1(SUR1)基因的外显子163c/t、内向整流性钾通道(potassium inwardlyrectifying channel subfamily J, member 11, KCNJ11 )基因的E23K变异与2型糖尿病(type 2 diabetes mellitus,T2DM)患者磺脲类药物继发性失效(secondary failure of sulfonylurea, SFS)的关系。方法选取山东地区 T2DM患者200例,其中磺脲类药物有效者114例,SFS者86例,运用SNaPshot技术(ABI Biosystem, USA)对SUR1 163c/t、KCNJ11 E23K进行基因分型。结果SUR1 163c/t和KCNJ11 E23K等位基因的发生频率均符合HardyWeinberg平衡;KCNJ11 E23K各基因型的分布在SFS组及有效组之间差异无统计学意义(P>005);SUR1 163c/t各基因型的分布在SFS组和有效组之间差异有统计学意义(P<001),且“t”等位基因的频率在SFS组明显增高[比值比(OR)=187,95%可信区间(CI)为123~285,P<001]。Logistic回归分析中,校正性别、年龄、BMI、FC肽、TG、TC、HDLC、LDLC后,SUR1 163c/t 的t/t基因型是SFS发生的独立危险因素[比值比(OR)=282,95%可信区间(CI)为157~507,P<001]。结论SUR1外显子 163c/t多态性可能与山东地区磺脲类药物继发性失效有明显相关性。
Abstract:
AimTo investigate whether the polymorphisms of exon 163c/t in SUR1 and E23K in KCNJ11 are correlated with secondary failure of sulfonylurea in type 2 diabetes mellitus. MethodsThe association of two SNPs in SUR1 and KCNJ11 was studied by genotyping them with ABI SNaPshot Multiplex System in 114 patients with sulfonylurea effective, and 86 patients with secondary failure of sulfonylurea. ResultsThe genotype frequencies distribution of KCNJ11 E23K were found to show no significant differences between SU failure group and SU effective group (P>005). There were significant differences in the distributive frequency of the SUR1 163 c/t polymorphism between SU failure group and SU effective group (P<001) . The risk allele “t” frequencies in SU failure group were significantly higher than that in SU effective group (odds ratio=187,95% confidence interval:123~285, P<001). Adjustment for age, gender, BMI, fasting Cpeptide, triglyceride, cholesterin, highdensity lipoprotein cholesterol, low density lipoprotein in cholesterol a logistic regression analysis did not change this association (odds ratio=282, 95% confidence interval:157~507, P<001). ConclusionThese data demonstrate that the polymorphism of exon 163c/t in SUR1 may be associated with secondary failure of sulfonylurea in type 2 diabetes mellitus in Shandong Province.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-02-03,修回日期:2013-04-01 基金项目:国家自然科学青年基金资助项目(No 30900828) 作者简介:邵倩(1986-),女,硕士生,研究方向:糖尿病磺脲类降糖药物基因组学,Tel:05372903177,Email:qian323pw@126.com; 班博(1963-),女,博士,教授,硕士生导师,研究方向:糖尿病病因学及并发症的防治,通讯作者,Tel:05372903077,Email:banbo2011@163.com; 施锦绣(1974-),女,博士,副研究员,研究方向:遗传学,通讯作者,Email:shijx@chgc.sh.cn
更新日期/Last Update: 2013-07-09