[1]肖 敏,王根柱,戚 欣,等.eEF2K对MDA-MB-231细胞增殖的影响与初步机制研究[J].中国药理学通报,2018,(08):1061-1065.[doi:10.3969/j.issn.1001-1978.2018.08.007]
 XIAO Min,WANG Gen-zhu,QI Xin,et al.Effect of eEF2K on proliferation of MDA-MB-231 and its preliminary mechanism[J].Chinese Pharmacological Bulletin,2018,(08):1061-1065.[doi:10.3969/j.issn.1001-1978.2018.08.007]
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eEF2K对MDA-MB-231细胞增殖的影响与初步机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1061-1065
栏目:
论著
出版日期:
2018-08-26

文章信息/Info

Title:
Effect of eEF2K on proliferation of MDA-MB-231 and its preliminary mechanism
文章编号:
1001-1978(2018)08-1061-05
作者:
肖 敏王根柱戚 欣李 静
中国海洋大学医药学院分子药理室,山东 青岛 266000
Author(s):
XIAO Min WANG Gen-zhu QI Xin LI Jing
Dept of Molecular Pharmacology, School of Medicine, Ocean University of China, Qingdao Shandong 266000,China
关键词:
eEF2K MDA-MB-231 增殖 信号通路 Akt mTOR
Keywords:
eEF2K MDA-MB-231 proliferation signaling pathways Akt mTOR
分类号:
R329.24; R345.57; R737.902.2; R977.3
DOI:
10.3969/j.issn.1001-1978.2018.08.007
文献标志码:
A
摘要:
目的 研究在不同培养条件下,真核延伸因子2激酶(eEF2K)对三阴性乳腺癌细胞MDA-MB-231增殖的影响,并初步探讨其机制。方法 慢病毒感染构建eEF2K表达抑制的MDA-MB-231稳定细胞株以及对应的空载体对照细胞株。在完全、无糖和无血清的培养条件下,SRB法测定细胞增殖曲线,平板克隆实验评价细胞的克隆形成能力,Western blot检测增殖相关蛋白的表达和活性。结果 贫营养条件下,抑制 MDA-MB-231细胞中eEF2K的表达能够促进细胞生长。而在完全培养条件下,抑制eEF2K表达则抑制细胞的增殖和克隆形成,并降低Akt和mTOR蛋白的活性,对其它增殖相关分子如PI3K、Src、ERK、JAK无明显影响。结论 不同培养条件下,eEF2K对MDA-MB-231细胞生长影响不同,其功能值得进一步研究。
Abstract:
Aim To investigate the effects of eukaryotic elongation factor 2 kinase(eEF2K)on the proliferation of triple negative breast cancer cell MDA-MB-231 under different culture conditions and to explore its preliminary mechanism.Methods The lentiviral was used to construct stably transfected cell lines.The cell proliferation curve was determined by SRB assay.The clonogenic capacity of cells was evaluated by plate clone assay.Moreover, to explore its mechanism, expressions and activities of proteins related to cell proliferation were estimated by Western blot assay.Results In complete medium, eEF2K inhibition of MDA-MB-231 cells promoted cell proliferation and colony formation, and down-regulated the activities of Akt and mTOR, rather than PI3K,Src,ERK and JAK.While in nutrient deprivation, inhibiting eEF2K resulted in promoted cell proliferation.Conclusion In different culture conditions, the expression of eEF2K in MDA-MB-231 has different effects on cell growth, and the role of eEF2K in cancer development remains to be explored.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-05-17,修回日期:2018-06-22
基金项目:国家自然科学基金资助项目(No 81673450)
作者简介:肖 敏(1993-),女,硕士生,研究方向:肿瘤分子药理学,E-mail:xm_1993@foxmail.com;
李 静(1968-),女,博士,教授,博士生导师,研究方向:肿瘤药理学,通讯作者,Tel:0532-82031980,E-mail:ljlilac@163.com
更新日期/Last Update: 2018-07-26