[1]娄 诤,赵肖涯,杜丽君,等.迷迭香酸抑制人多发性骨髓瘤ARH-77细胞增殖的作用机制[J].中国药理学通报,2018,(08):1078-1082.[doi:10.3969/j.issn.1001-1978.2018.08.010]
 LOU Zheng,ZHAO Xiao-ya,DU Li-jun,et al.Mechanism of rosmarinic acid inhibiting proliferation of human multiple myeloma ARH-77 cells[J].Chinese Pharmacological Bulletin,2018,(08):1078-1082.[doi:10.3969/j.issn.1001-1978.2018.08.010]
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迷迭香酸抑制人多发性骨髓瘤ARH-77细胞增殖的作用机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1078-1082
栏目:
论著
出版日期:
2018-07-26

文章信息/Info

Title:
Mechanism of rosmarinic acid inhibiting proliferation of human multiple myeloma ARH-77 cells
文章编号:
1001-1978(2018)08-1078-05
作者:
娄 诤1赵肖涯2杜丽君2王大维3
浙江中医药大学 1.第二临床医学院、 2.生命科学学院、3.基础医学院,浙江 杭州 310053
Author(s):
LOU Zheng1ZHAO Xiao-ya2DU Li-jun2WANG Da-wei3
1.The Second Clinical Medical School,2.School of Life Sciences,3.Basic Medical School,Zhejiang Chinese Medical University,Hangzhou 310053,China
关键词:
迷迭香酸 ARH-77细胞 tBid Fas caspase-3 caspase-9 caspase-8
Keywords:
rosmarinic acid ARH-77 cells tBid Fas caspase-3 caspase-9 caspase-8
分类号:
R284.1; R329.24; R345.79; R394.2; R733.302.2; R977.3; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.08.010
文献标志码:
A
摘要:
目的 研究迷迭香酸(rosmarinic acid,RosA)对ARH-77细胞的抑制作用及其机制。方法 采用CCK-8法检测RosA对ARH-77细胞增殖的影响,并由该结果确定实验组用药浓度分别为:20、40、80 μmol·L-1,作用时间48 h; 通过流式细胞术检测凋亡率; 采用qPCR法检测Fas、caspase-8、caspase-9 mRNA表达; 采用分光光度法检测caspase-8的活性; Western blot法检测tBid、Fas、活性caspase-3和活性caspase-9的蛋白水平。结果 RosA能明显抑制ARH-77细胞的增殖; 与对照组相比:各实验组均主要发生早期凋亡(P<0.01); 各实验组活性的caspase-3、caspase-9水平均明显升高(P<0.01),caspase-8 mRNA表达也明显上调,但其蛋白活性仅在RosA 80 μmol·L-1时明显升高(P<0.01); 在RosA(40、80 μmol·L-1)组,caspase-9 mRNA表达明显上调(P<0.05,P<0.01),tBid的蛋白表达也明显升高(P<0.01); Fas mRNA和蛋白表达仅在RosA 80 μmol·L-1组明显升高(P<0.01)。结论 RosA诱导ARH-77细胞早期凋亡而导致了增殖抑制,死亡受体途径和线粒体途径可能共同参与了RosA诱导的ARH-77细胞凋亡。
Abstract:
Aim To study the inhibitory effect of rosmarinic acid(RosA)on ARH-77 cells and its mechanism.Methods The effect of RosA on the proliferation of ARH-77 cells was detected by CCK-8 method.The results confirmed that the concentration of the drug in the experimental group was 20,40 and 80 μmol·L-1,respectively,for 48 h; the apoptosis rate was detected by flow cytometry; mRNA expression of Fas,caspase-8,and caspase-9 was measured by qPCR; the activity of caspase-8 was measured by spectrophotometry; tBid,Fas,active caspase-3,and active caspase-9 were detected by Western blot.Results RosA obviously inhibited the proliferation of ARH-77 cells.Compared with control group,early apoptosis was observed in all experimental groups(P<0.01).In each experimental group,the levels of active caspase-3 and caspase-9 markedly increased(P<0.01),caspase-8 mRNA expression was also significantly up-regulated,but its protein activity only evidently increased at 80 μmol·L-1(P<0.01); at 40 and 80 μmol·L-1 groups,the mRNA expression of caspase-9 was significantly up-regulated(P<0.05,P<0.01),and the protein expression of tBid also notably increased(P<0.01); the mRNA and protein expression of Fas dramatically increased only at 80 μmol·L-1 group(P<0.01).Conclusions The early apoptosis of ARH-77 cells induced by RosA leads to the inhibition of proliferation.The death receptor and mitochondrial pathways may participate in the apoptosis of ARH-77 cells induced by RosA.

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备注/Memo

备注/Memo:
收稿日期:2018-03-30,修回日期:2018-04-30
基金项目:国家自然科学基金面上项目(No 81373633); 浙江省自然科学基金资助项目(No LY18H270014)
作者简介:娄 诤(1996-),女,研究方向:肿瘤的防治及机制,E-mail:920069840@qq.com;
王大维(1980-),男,硕士,助理实验师,研究方向:中药药理学,通讯作者,E-mail:602599510@qq.com
更新日期/Last Update: 2018-07-26