[1]王 芳,孙耀贵,尹 伟,等.苦参碱联合黄芩苷对LPS诱导小鼠肺炎的影响[J].中国药理学通报,2018,(08):1105-1109.[doi:10.3969/j.issn.1001-1978.2018.08.015]
 WANG Fang,SUN Yao-gui,YIN Wei,et al.Effects of matrine combined with baicalin on mouse pneumonia induced by LPS[J].Chinese Pharmacological Bulletin,2018,(08):1105-1109.[doi:10.3969/j.issn.1001-1978.2018.08.015]
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苦参碱联合黄芩苷对LPS诱导小鼠肺炎的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1105-1109
栏目:
论著
出版日期:
2018-08-26

文章信息/Info

Title:
Effects of matrine combined with baicalin on mouse pneumonia induced by LPS
文章编号:
1001-1978(2018)08-1105-05
作者:
王 芳孙耀贵尹 伟范阔海段智变孙 娜李宏全
山西农业大学动物科技学院,山西 太谷 030801
Author(s):
WANG Fang SUN Yao-gui YIN Wei FAN Kuo-hai DUAN Zhi-bian SUN Na LI Hong-quan
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu Shanxi 030801, China
关键词:
苦参碱 黄芩苷 小鼠 肺炎 LPS IL-6 TNF-α
Keywords:
matrine baicalin mice pneumonia LPS IL-6 TNF-α
分类号:
R-332; R284.1; R322.35; R392.12; R563.105.31
DOI:
10.3969/j.issn.1001-1978.2018.08.015
文献标志码:
A
摘要:
目的 研究苦参碱与黄芩苷联合作用对LPS诱导的小鼠肺炎的影响。方法 将42只SPF♀小鼠随机分成空白组、LPS组、苦参碱组、黄芩苷组、联合高剂量组、联合中剂量组和联合低剂量组,每组6只。腹腔注射20 mg·kg-1 LPS制备小鼠肺炎模型,在感染30 min后,腹腔注射不同剂量的药物。于感染24 h后处死小鼠,采集血液和肺组织。HE染色观察小鼠肺组织病理学变化; ELISA法检测血清中IL-6和TNF-α含量; qPCR检测肺组织中IL-6、TNF-α mRNA的表达; MPO检测试剂盒检测肺脏MPO活性。结果 与空白组相比,LPS组小鼠出现肺泡间隔增厚、炎性细胞渗出、充血等肺炎症状,血清和肺组织中炎症因子IL-6和TNF-α表达量明显升高(P<0.01),MPO活性明显增加(P<0.01)。与LPS组相比,用药组小鼠肺组织病变减轻,肺组织IL-6、TNF-α mRNA表达明显降低(P<0.01),MPO活性也明显降低(P<0.05)。结果表明苦参碱和黄芩苷有抗炎作用,且7.5 mg·kg-1苦参碱和50 mg·kg-1黄芩苷联合使用的药效与30 mg·kg-1苦参碱或200 mg·kg-1黄芩苷单独使用时一致。结论 苦参碱、黄芩苷和二者联用均表现出较好的抑制炎症作用,而联合低剂量组用药量最少,效果和单独用药组差异无显著性,提示低剂量苦参碱和黄芩苷联用效果较好。
Abstract:
Aim To investigate the effects of matrine combined with baicalin on lipopolysaccharide(LPS)induced lung injury in mice.Methods Forty-two mice were randomly divided into seven groups, including control group, LPS group, matrine group, baicalin group, combined high dose group, combined middle dose group and combined low dose group.A mouse model of acute lung injury was established by intraperitoneal injection of 20 mg·kg-1 LPS.Various concentrations of baicalin, matrine and matrine combined with baicalin were injected intraperitoneally 30 min after the administration of LPS.After 24 h of LPS challenge, serum was collected to examine the levels of interleukin(IL)-6 and tumor necrosis factor(TNF)-α by ELISA.The lung tissues were isolated for observing pathological changes by HE staining and measuring the IL-6 and TNF-α mRNA levels by qPCR.MPO activity was determined by MPO activity kit.Results The intraperitoneal administration of LPS significantly damaged pulmonary function and induced inflammatory cell infiltration into the lungs.Compared with control group, the levels of IL-6 and TNF-α in both serum and lung tissues of LPS group increased(P<0.01).Compared with LPS group, pulmonary function in compound treatment groups was improved, the levels of IL-6 and TNF-α in lung tissues significantly decreased(P<0.01), and the levels in serum showed a downward trend.The MPO activities in compound treatment groups were reduced(P<0.05).Conclusions Both matrine and baicalin possess anti-inflammation effects and no significant difference exists between 7.5 mg·kg-1 matrine combined with 50 mg·kg-1 baicalin and 30 mg·kg-1 matrine alone group or 200 mg·kg-1 baicalin alone group.Considering with the dosage and efficacy, the low dose of matrine combined with baicalin has more economic benefit and lower toxicity, as well as the minimum cost than other groups, hence it is recommended in future clinical application.

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备注/Memo

备注/Memo:
收稿日期:2018-04-18,修回日期:2018-05-14
基金项目:国家重点研发计划项目(No 2017YFD0501500); 山西农业大学科技创新基金资助项目(No 2017001)
作者简介:王 芳(1991-),女,硕士生,研究方向:中药调节动物免疫功能及其分子机制,E-mail: 401954789@qq.com;
孙 娜(1986-),女,博士,讲师,硕士生导师,研究方向:中药调节动物免疫功能及其分子机制,通讯作者,E-mail: sxndsn@126.com;
李宏全(1963-),男,博士,教授,博士生导师,研究方向:中药调节动物免疫功能及其分子机制,通讯作者,E-mail: livets@163.com
更新日期/Last Update: 2018-07-26