[1]高 峰,卢伟龙,王华林,等.大鼠背根神经节细胞中TRPV1与cAMP/PKA通路调控机制研究[J].中国药理学通报,2018,(08):1110-1114.[doi:10.3969/j.issn.1001-1978.2018.08.016]
 GAO Feng,LU Wei-long,WANG Hua-lin,et al.Study on regulatory mechanism of TRPV1 and cAMP/PKA pathway in dorsal root ganglion cells of rats[J].Chinese Pharmacological Bulletin,2018,(08):1110-1114.[doi:10.3969/j.issn.1001-1978.2018.08.016]
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大鼠背根神经节细胞中TRPV1与cAMP/PKA通路调控机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1110-1114
栏目:
论著
出版日期:
2018-07-26

文章信息/Info

Title:
Study on regulatory mechanism of TRPV1 and cAMP/PKA pathway in dorsal root ganglion cells of rats
文章编号:
1001-1978(2018)08-1110-05
作者:
高 峰卢伟龙王华林吴媛媛王 斌李 敏
陕西中医药大学药学院,陕西 西安 712000
Author(s):
GAO Feng LU Wei-long WANG Hua-lin WU Yuan-yuan WANG Bin LI Min
College of Pharmaceutical Science,Shanxi University of Chinese Medicine, Xi'an 712000,China
关键词:
大鼠背根神经节 原代细胞 TRPV1 cAMP/PKA通路 磷酸化 调控
Keywords:
rat dorsal root ganglion primary cell TRPV1 cAMP/PKA pathway phosphorylation regulation
分类号:
R-332; R322.8; R329.24; R342.4; R392.11; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.08.016
文献标志码:
A
摘要:
目的 探讨大鼠背根神经节细胞中,辣椒素受体(transient receptor potential vanilloid 1,TRPV1)与体内cAMP/PKA信号通路的调控作用。方法 分离培养大鼠背根神经节原代细胞,分别应用cAMP/PKA信号通路的激动剂8-Br-cAMP和抑制剂H-89,TRPV1的激动剂辣椒素(capsaicin)、抑制剂i RTX进行干预,应用定量PCR和Western blot检测各组细胞的TRPV1、p-PKA的基因和蛋白表达,探讨TRPV1与体内cAMP/PKA信号通路之间的调控机制。结果 与空白对照组比较,8-Br-cAMP组、capsaicin组TRPV1的基因及蛋白表达均明显增强,i RTX组TRPV1的基因及蛋白表达明显降低,H-89组TRPV1基因、蛋白的表达无明显变化; 8-Br-cAMP组p-PKA的基因及蛋白表达明显增强,H-89组p-PKA的基因及蛋白表达明显降低,而capsaicin组、i RTX组p-PKA基因、蛋白的表达无明显变化。结论 cAMP-PKA信号通路是调控TRPV1表达的重要作用机制之一,同时可能存在其他信号通路对TRPV1也有一定调控作用。
Abstract:
Aim To investigate the regulation of TRPV1 and cAMP/PKA signal pathway in rat dorsal root ganglion(DRG)cells.Methods Primary cells of rat DRG were isolated and treated with 8-Br-cAMP and H-89, agonist and inhibitor of cAMP/PKA signaling pathway, capsaicin and i RTX, agonist and inhibitor of TRPV1.The expression of TRPV1 and p-PKA in each group of cells was detected by qPCR and Western blot.The regulatory mechanism of TRPV1 and cAMP/PKA signaling pathway was explored.Results Compared with the blank control group, the gene and protein expression of TRPV1 in capsaicin group significantly increased, while the gene and protein expression of TRPV1 in i RTX group significantly decreased.However, the expression of p-PKA gene and protein showed no significant change.The gene and protein expressions of p-PKA and TRPV1 in 8-Br-cAMP group were significantly enhanced, and the gene and protein expressions of p-PKA and TRPV1 in H-89 group significantly decreased.Conclusions The cAMP-PKA signaling pathway is one of the most important mechanisms regulating the expression of TRPV1.At the same time, other signaling pathways may have some regulatory roles in TRPV1.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-03-17,修回日期:2018-04-22
基金项目:国家自然科学基金资助项目(No 81303243); 陕西省中医药管理局项目(No JCPT007)
作者简介:高 峰(1982-),女,博士,讲师,研究方向:中药毒性,Tel:029-38185165,E-mail:7204612@qq.com;
李 敏(1978-),女,博士,教授,研究方向:中药基础理论,通讯作者,Tel:029-38185165,E-mail:liminsweet@126.com
更新日期/Last Update: 2018-07-26