[1]王盈盈,刘 青,唐丽琴,等.小檗碱对高糖诱导足细胞功能及相关蛋白表达的影响[J].中国药理学通报,2018,(08):1158-1163.[doi:10.3969/j.issn.1001-1978.2018.08.024]
 WANG Ying-ying,LIU Qing,et al.Effect of berberine on high glucose induced podocyte functions and related protein expression[J].Chinese Pharmacological Bulletin,2018,(08):1158-1163.[doi:10.3969/j.issn.1001-1978.2018.08.024]
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小檗碱对高糖诱导足细胞功能及相关蛋白表达的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1158-1163
栏目:
论著
出版日期:
2018-07-26

文章信息/Info

Title:
Effect of berberine on high glucose induced podocyte functions and related protein expression
文章编号:
1001-1978(2018)08-1158-06
作者:
王盈盈12刘 青12唐丽琴12魏 伟1
1.安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,抗炎免疫药物安徽省协同创新中心,安徽 合肥 230032; 2.安徽医科大学附属省立医院药剂科,安徽 合肥 230001
Author(s):
WANG Ying-ying1 2 LIU Qing1 2 TANG Li-qin1 2 WEI Wei1
1.Institute of Clinical Pharmacology, Anhui Medical University, Key Lab of Anti-inflammatory and Immune Medicine, Ministry of Education, Collaborative Innovation Center of Anti-inflammatory and Immune Medicines, Hefei 230032, China; 2.Dept of Pharmacy, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China
关键词:
小檗碱 糖尿病肾病 足细胞 凋亡 nephrin podocin
Keywords:
berberine diabetic nephropathy podocytes apoptosis nephrin podocin
分类号:
R-332; R287; R322.61; R329.25; R692.390.22; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.08.024
文献标志码:
A
摘要:
目的 探讨小檗碱对高糖诱导肾小球足细胞损伤的保护作用及其机制。方法 体外培养小鼠肾小球足细胞,分为正常对照组、高糖模型组、不同浓度小檗碱组(30、60、90 μmol·L-1),分别处理足细胞。MTT法检测小檗碱对高糖诱导足细胞增殖能力的影响; Annexin V-FITC/PI双染实验观察小檗碱对高糖诱导足细胞凋亡的影响; 利用Transwell小室观察小檗碱对高糖诱导足细胞迁移能力的影响; Western blot法检测小檗碱对高糖诱导足细胞nephrin、podocin、desmin蛋白表达的影响; 黏附能力实验检测小檗碱对高糖诱导足细胞黏附功能的影响。结果 MTT检测结果显示,小檗碱能增加高糖诱导足细胞的活力; Annexin V-FITC/PI双染实验结果显示,小檗碱能抑制高糖诱导足细胞的凋亡; Transwell迁移实验结果显示,小檗碱可以抑制高糖诱导足细胞的迁移; Western blot结果显示,小檗碱可降低高糖诱导足细胞desmin蛋白表达,明显升高nephrin与podocin蛋白的表达; 黏附功能实验结果表明,小檗碱能明显提高高糖诱导足细胞的黏附能力。结论 小檗碱改善高糖诱导足细胞功能的损伤,其作用机制可能与保护足细胞nephrin和podocin蛋白相关。
Abstract:
Aim To investigate the protective effect of berberine on high glucose(HG)induced podocytes injury and the detailed mechanism.Methods Mouse glomerular podocytes were cultured in vitro and divided into control group, HG model group, and different concentrations of berberine(30, 60, 90 μmol·L-1)treated group respectively.The cell proliferation was detected by MTT assay; the apoptosis was detected by Annexin V-FITC/PI double staining; the ability of migration was observed by transwell assay; the expressions of nephrin, podocin and desmin were detected by Western blot; the adhesion function was detected by adhesion ability assay.Results The HG-induced podocyte viability was significantly promoted by berberine(30, 60, 90 μmol·L-1).The apoptosis and migration of HG-induced podocyte significantly decreased after exposure to berberine.After treatment with berberine(30, 60, 90 μmol·L-1)in HG-induced podocyte, the expression of nephrin and podocin markedly increased, while desmin expression significantly decreased.Meanwhile, the adhesion ability increased after treated with berberine.Conclusion Berberine improves the damage of podocyte induced by HG, which may be related to the protection of nephrin and podocin in podocytes.

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备注/Memo

备注/Memo:
收稿日期:2018-03-16,修回日期:2018-04-21
基金项目:国家自然科学基金资助项目(No 81773955); 安徽省自然科学基金资助项目(No 1508085MH179); 安徽省中医药科研课题(No 2016zy08)
作者简介:王盈盈(1993-),女,硕士生,研究方向:内分泌及代谢药理学,E-mail:wangyingying601@163.com;
唐丽琴(1972-),女,博士,主任药师,教授,硕士生导师,研究方向:内分泌及代谢药理学,通讯作者,E-mail:tulcyl@sina.com;
魏 伟(1960-),男,博士,教授,博士生导师,研究方向:抗炎免疫药理学,通讯作者,E-mail:wwei@ahmu.edu.cn
更新日期/Last Update: 2018-07-26