[1]吴 斌,张 一,陈 勇,等.丹蛭降糖胶囊治疗肥胖引起的慢性肾病的机制研究[J].中国药理学通报,2018,(08):1163-1169.[doi:10.3969/j.issn.1001-1978.2018.08.025]
 WU Bin,ZHANG Yi,CHEN Yong,et al.The preliminary mechanism of Danzhi Jiangtang Capsule for improving high fat diet-induced kidney damages[J].Chinese Pharmacological Bulletin,2018,(08):1163-1169.[doi:10.3969/j.issn.1001-1978.2018.08.025]
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丹蛭降糖胶囊治疗肥胖引起的慢性肾病的机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1163-1169
栏目:
复方药物药理学
出版日期:
2018-08-26

文章信息/Info

Title:
The preliminary mechanism of Danzhi Jiangtang Capsule for improving high fat diet-induced kidney damages
文章编号:
1001-1978(2018)08-1163-07
作者:
吴 斌12张 一3陈 勇1刘 权4李 瑞4胡冰峰4方朝晖5鲁云霞1
1.安徽医科大学生物化学教研室,安徽 合肥 230032; 2.安徽卫生健康职业学院,安徽 池州 247099; 3.安徽医科大学附属第一医院内分泌科,安徽 合肥 230022; 4.安徽医科大学药学院临床药学专业2014级,安徽 合肥 230032; 5.安徽中医药大学附属第一医院内分泌科,安徽 合肥 230031
Author(s):
WU Bin12 ZHANG Yi3 CHEN Yong1 LIU Quan4 LI Rui4 HU Bing-feng4 FANG Zhao-hui5 LU Yun-xia1
1.Dept of Biochemistry and Molecular Biology, Anhui Medical University, Hefei 230032, China; 2.Anhui Hygiene and Health Professional School, Chizhou Anhui 247099,China; 3.Dept of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China;...
关键词:
丹蛭降糖胶囊 高脂血症 慢性肾病 氧化应激 AMPK 脂代谢
Keywords:
Danzhi Jiangtang Capsule hyperlipidemia chronic kidney disease oxidative stress AMPK lipid metabolism
分类号:
R-332; R285.5; R287; R322.61; R589.2; R692.02; R692.053.1
DOI:
10.3969/j.issn.1001-1978.2018.08.025
文献标志码:
A
摘要:
目的 探究丹蛭降糖胶囊(DJC)改善肥胖引起的大鼠慢性肾病的初步机制。方法 ♂ SD大鼠随机分为基础饲料组(CON)、高脂饲料组(HFD)、高脂饲料+丹蛭降糖胶囊低剂量组(HFD+DJCL,500 mg·kg-1·d-1)、高脂饲料+丹蛭降糖胶囊高剂量组(HFD+DJCH,1 000 mg·kg-1·d-1),除CON组外,均给予高脂饮食12周,治疗组再灌胃治疗8周。检测血清血脂和肾代谢指标, HE、油红O、PAS染色观察肾脏的病理学变化,10%肾匀浆测定肾组织TG、TSOD、CuZn-SOD、CAT、GSH-Px、TNOS、MDA含量,免疫组化法检测CD36的表达变化,RT-PCR分析SREBP1c及FASN的表达,Western blot分析DJC对AMPK、磷酸化AMPK、PPARα、PPARγ表达的影响。结果 对比CON组,HFD组大鼠体质量增加,血清TC、TG、UA、BUN、Scr升高,HDL-C下降,肾小球出现明显的损伤及脂质沉积,GSH-Px、MDA升高,TSOD、CAT、CuZn-SOD、TNOS水平降低,CD36、SREBP1c、FASN、PPARγ的表达上升,PPARα、AMPK、磷酸化AMPK表达下降; 对比HFD组,HFD+DJCL和HFD+DJCH组均明显逆转上述指标,且有一定的剂量效应。结论 DJC可有效降低高脂饮食引起的肾内脂质沉积和氧化应激水平,其机制可能是通过激活AMPK-PPARα通路,抑制CD36和脂肪生成基因PPARγ、SREBP1c、FASN的表达,并有一定的剂量效应。
Abstract:
Aim To study the preliminary mechanism of Danzhi Jiangtang Capsule(DJC)on the high-fat diet-induced injury in kidney of rats.Methods Male SD rats were randomly divided into control diet group(CON), high-fat diet group(HFD), HFD and DJC low dose treatment group(HFD+DJCL), HFD and DJC high dose treatment group(HFD+DJCH).The latter three groups were fed HFD for 12 weeks, then the latter two groups were given DJC 500 mg·kg-1·d-1 and 1 000 mg·kg-1·d-1 via gavage for 8 weeks respectively.The levels of serum lipid and kidney metabolism indices were detected.HE, Oil red O and PAS staining were used to observe the pathological changes of kidney.10% kidney homogenate was used to detect triglyceride(TG), total superoxide dismutase(TSOD), CuZn superoxide dismutase(CuZn-SOD), catalase(CAT), glutathione peroxidase(GSH-Px), total nitric oxide synthase(TNOS), malonaldehyde(MDA).Expression and distribution of FAT/CD36 were detected using immunohistochemistry.Expression of SREBP1c and FASN were detected using RT-PCR.Expression of AMPK, phosphorylation of AMPK, PPARα, PPARγ were analyzed using Western blot.Results Compared with CON group rats, rats in HFD group showed greater weight, higher serum levels of TC, TG, UA, Scr, BUN, and lower serum levels of HDL-C.Obvious injury and lipid deposition were observed in the kidney, GSH-Px and MDA content increased, meanwhile TSOD, CuZn-SOD, CAT, TNOS levels decreased.Expression of FAT/CD36, SREBP1c, FASN and PPARγ increased, while that of PPARα, AMPK and phosphorylation of AMPK decreased.Compared with HFD group, the HFD+DJC groups showed significantly improved above indices in a somewhat dose-dependent manner.Conclusions DJC effectively reduces HFD-induced lipid deposition and oxidative stress in kidney, and the mechanisms are possibly related with activated AMPK-PPARα signaling and decreased expression of CD36 and lipogenic genes PPARγ, SREBP1c, FASN, exhibiting a dose-dependent effect.

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备注/Memo

备注/Memo:
收稿日期:2018-04-15,修回日期:2018-05-15
基金项目:国家中医临床研究基地业务建设专项课题(No JDZX2015129); 安徽省高等学校自然科学研究项目(No KJ2017A186); 地方高校国家级大学生创新创业训练计划项目(No 201710366019)
作者简介:吴 斌(1982-),男,硕士生,研究方向:中药药理学,E-mail:wbing1030@163.com;
鲁云霞(1969-),女,博士,副教授,硕士生导师,研究方向:代谢性疾病机制及治疗,通讯作者,E-mail: wwwdluyx@sina.com
更新日期/Last Update: 2018-07-26