[1]朱 晨,刘 伟,李 璟,等.酪氨酸羟化酶与钩藤碱抑制甲基苯丙胺依赖小鼠条件性位置偏爱的关系研究[J].中国药理学通报,2018,(09):1226-1230.[doi:10.3969/j.issn.1001-1978.2018.09.009]
 ZHU Chen,LIU Wei,LI Jing,et al.Effect of rhynchophylline on expression of TH in methamphetamine-induced CPP mice[J].Chinese Pharmacological Bulletin,2018,(09):1226-1230.[doi:10.3969/j.issn.1001-1978.2018.09.009]
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酪氨酸羟化酶与钩藤碱抑制甲基苯丙胺依赖小鼠条件性位置偏爱的关系研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1226-1230
栏目:
论著
出版日期:
2018-08-26

文章信息/Info

Title:
Effect of rhynchophylline on expression of TH in methamphetamine-induced CPP mice
文章编号:
1001-1978(2018)09-1226-05
作者:
朱 晨1刘 伟1李 璟2李 婵1房 淼1陈志杰1周玉婷1罗超华1莫志贤1
南方医科大学1.中医药学院中药药理学教研室、2.中心实验室,广东 广州 510515
Author(s):
ZHU Chen1 LIU Wei1 LI Jing2 LI Chan1 FANG Miao1 CHEN Zhi-jie1 ZHOU Yu-ting1 LUO Chao-hua1 MO Zhi-xian1
1.Dept of Traditional Chinese Medicine Pharmacology, School of Traditional Chinese Medicine; 2.Central Lab, Southern Medical University, Guangzhou 510515, China
关键词:
钩藤碱 甲基苯丙胺 条件性位置偏爱 药物依赖 酪氨酸羟化酶 氯胺酮
Keywords:
rhynchophylline methamphetamine condition place preference drug addiction tyrosine hydroxylase katamine
分类号:
R-332; R284.1; R322.81; R749.61; R971.7; R971.2; R977.3; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.09.009
文献标志码:
A
摘要:
目的 研究酪氨酸羟化酶(tyrosine hydroxylase, TH)与钩藤碱抑制甲基苯丙胺依赖小鼠条件性位置偏爱(condition place preference, CPP)作用的关系。方法 实验分为5组:空白对照组、模型组、钩藤碱低剂量组(40 mg·kg-1)、钩藤碱高剂量组(80 mg·kg-1)、氯胺酮组(15 mg·kg-1)。腹腔注射甲基苯丙胺后,进行小鼠条件性位置偏爱训练,建立小鼠甲基苯丙胺CPP依赖模型,分析各组小鼠在CPP箱中的活动,以及各组小鼠脑内TH 的表达情况。结果 给予甲基苯丙胺4 mg·kg-1后,甲基苯丙胺依赖小鼠CPP模型成功建立。与空白组相比,模型组小鼠在伴药箱中的时间明显增加; 与模型组相比,钩藤碱组小鼠在伴药箱中的时间明显减少。与空白组相比,模型组小鼠脑内TH阳性细胞数明显增加; 在给予钩藤碱干预后,小鼠脑内TH阳性细胞数明显减少。与空白组比较,模型组小鼠脑内TH蛋白表达明显增强,给予钩藤碱干预后,TH蛋白表达减弱。 结论 钩藤碱在一定程度上可以抑制甲基苯丙胺诱导的小鼠CPP效应,其作用机制可能与TH的表达有关。
Abstract:
Aim To explore the effect of rhynchophylline on the expression of tyrosine hydroxylase(TH)in hippocampus of methamphetamine-induced condition place preference(CPP)mice. Methods Metham-phetamine was injected intraperitoneally to mice, and the expression of TH was observed by immunohistochemistry and Western blot. Results The CPP mouse model was established successfully by methamphetamine(4 mg·kg-1). Ketamine(15 mg·kg-1), rhynchophylline low dosage group(40 mg·kg-1)and rhynchophylline high dosage group(80 mg·kg-1)could remove the effect of methamphetamine on CPP mice. The result of immunohistochemistry showed that methamphetamine(4 mg·kg-1)could increase the number of TH positive cells in hippocampus while ketamine(4 mg·kg-1), rhynchophylline(40, 80 mg·kg-1)group could attenuate the change. Western blotting indicated the expression of TH of model group increased significantly, whereas ketamine(15 mg·kg-1), rhynchophylline(40, 80 mg·kg-1)group presented less expression. Conclusions The CPP induced by methamphetamine in mice may be inhibited to some extent by rhynchophylline, and its mechanism may be associated with the expression of TH.

参考文献/References:

[1] Ndagijimana A, Wang X, Pan G, et al. A review on indole alkaloids isolated from Uncaria rhynchophylla and their pharmacological studies[J]. Fitoterapia, 2013, 86: 35-47.
[2] 汪江涛, 丁伯平.钩藤碱和异钩藤碱药理作用研究进展[J].中国医药刊,2012,14(7):1224-5,1221.
[2] Wang J T, Ding B P. Progress in research of rhynchophylline and isorhynchophylline on pharmacological[J].Chin J Med Guide, 2012, 14(7): 1224-5, 1221.
[3] Tokuoka H, Muramatsu S I, Sumi-Ichinose C, et al. Compensatory regulation of dopamine after ablation of the tyrosine hydroxylase gene in the nigrostriatal projection[J]. J Biol Chem, 2011,286(50):43549-58.
[4] 张 阳,张志坚,俞晓岚, 等. 慢病毒介导的新型Tet-On系统调控大鼠GDNF和TH双基因表达对帕金森病大鼠的实验研究[J].中国药理学通报, 2015,31(2):251-6.
[4] Zhang Y, Zhang Z J, Yu X L, et al. Experimental study on expression of TH and GDNF bi-gene of lentivirus by improved Tet-On system for a rat model of Parkinson's disease[J].Chin Pharmacol Bull,2015,31(2):251-6.
[5] 武变瑛,李 娜,刘秀平. 复方石菖蒲合剂对吗啡依赖小鼠位置偏爱效应及血中单胺类递质的影响[J].中国疼痛医学杂志,2009, 15(5):283-6.
[5] Wu B Y, Li N, Liu X P, et al. Effect of acrous-tatrinown-schott mixture on morphine-induced place preference and blood monoamine neurotransmitters in mice[J]. Chin J Pain Med, 2009, 15(5):283-6.
[6] 邓慧琼,周旭辉,张登科, 等. 条件性位置实验方法及非倾向性实验设计在药物依赖研究中的应用[J].中国药物依赖性杂志,2006,15(2):8-11.
[6] Deng H Q, Zhou X H, Zhang D K, et al. The application of conditional location experiment method and non-biased experiment design in drug dependence research[J]. Chin J Drug Depend, 2006,15(2):8-11.
[7] Volkoff H. The effects of amphetamine injections on feeding behavior and the brain expression of orexin, CART, tyrosine hydroxylase(TH)and thyrotropin releasing hormone(TRH)in goldfish(Carassius auratus)[J]. Fish Physiol Biochem, 2013,39(4):979-91.
[8] Zhu C, Liu W, Luo C H, et al. Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase(TH)[J]. Fitoterapia, 2017,117:47-51.
[9] Park S W, He Z, Shen X, et al. Differential action of methamphetamine on tyrosinehydroxylase and dopamine transport in the nigrostriatal pathway of mu-opioid receptor knockout mice[J]. Int J Neurosci, 2012, 122(6):305-13.
[10] Jiang M J, Chen Y F, Li C, et al. Inhibiting effects of rhynchophylline on zebrafish methamphetamine dependence are associated with amelioration of neurotransmitters content and down-regulation of TH and NR2B expression[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2016,68:31-43.
[11] 周吉银,莫志贤.钩藤碱对苯丙胺依赖大鼠伏核和杏仁核中NR2B蛋白表达的影响[J].中国药物依赖性杂志,2007,16(1):12-9.
[11] Zhou J Y, Mo Z X. The effect of rhynchophylline on NR2B expression in nucleus accubens and amygdaloid of amphetamine-dependent rats[J]. Chin J Drug Depend, 2007, 16(1):12-9.
[12] 周吉银,莫志贤.钩藤碱对苯丙胺依赖大鼠神经核团中NR2B mRNA表达的影响[J].中国药理学通报,2007,23(9):1141-5.
[12] Zhou J Y, Mo Z X. The effect of rhynchophylline on NR2B mRNA expression in nucleus of amphetamine-dependent rats[J]. Chin Pharmacol Bull,2007,23(9):1141-5.
[13] Zhou J, Chen J, Zhou S, et al. Individual an combined effects of ehychophylline and ketamine on proliferation, NMDAR1 and GluA2/3 protein expression in PC12 cells[J].Fitoterapia, 2013,85:125-9.
[14] Liu W, Peng Q, Lin X, et al. Effect of rhynchophylline on the expression of p-CREB and sc-Fos in triatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference rats[J]. Fitoterapia, 2014,92:16-22.

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备注/Memo

备注/Memo:
收稿日期:2018-05-18,修回日期:2018-06-24
基金项目:国家自然科学基金资助项目(No 81673628, 81573706)
作者简介:朱 晨(1994-),女,博士生,研究方向:中药药理学,E-mail:834927604@qq.com;
莫志贤(1958-),女,硕士,教授,研究方向:中药药理学,通讯作者,E-mail: cherrymo@fimmu.com
更新日期/Last Update: 2018-08-26