[1]鲍 宁,马 虹.七氟烷预处理上调miR-199a-5p对脊髓缺血/再灌注损伤的保护作用[J].中国药理学通报,2018,(09):1230-1234.[doi:10.3969/j.issn.1001-1978.2018.09.010]
 BAO Ning,MA Hong.Sevoflurane preconditioning protects against spinal cord ischemia/reperfusion injury by up-regulating miR-199a-5p in rats[J].Chinese Pharmacological Bulletin,2018,(09):1230-1234.[doi:10.3969/j.issn.1001-1978.2018.09.010]
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七氟烷预处理上调miR-199a-5p对脊髓缺血/再灌注损伤的保护作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1230-1234
栏目:
论著
出版日期:
2018-08-26

文章信息/Info

Title:
Sevoflurane preconditioning protects against spinal cord ischemia/reperfusion injury by up-regulating miR-199a-5p in rats
文章编号:
1001-1978(2018)09-1230-05
作者:
鲍 宁12马 虹1
1.中国医科大学附属第一医院麻醉科,辽宁 沈阳 110001; 2.沈阳市妇婴医院麻醉科,辽宁 沈阳 110003
Author(s):
BAO Ning12 MA Hong1
1.Dept of Anaesthesiology, the First Hospital of China Medical University, Shenyang 110001, China; 2.Shenyang Women's and Children's Hospital, Shenyang 110003, China
关键词:
七氟烷预处理 缺血/再灌注损伤 脊髓 miR-199a-5p 凋亡 血脊屏障
Keywords:
sevoflurane preconditioning ischemia-reperfusion injury spinal cord miR-199a-5p apoptosis blood-spinal cord barrier
分类号:
R-332; R322.81; R329.25; R342.2; R619.9; R744.1; R971.2
DOI:
10.3969/j.issn.1001-1978.2018.09.010
文献标志码:
A
摘要:
目的 研究miR-199a-5p在七氟烷预处理大鼠脊髓缺血/再灌注损伤(SCIRI)中的作用。方法 将24只SD 大鼠随机分为:假手术组(Sham组)、模型组(I/R组)、七氟烷+模型组(SEVO+I/R组)。Sham组给予同样的手术,但不吸入七氟烷或夹闭主动脉弓; I/R组无创动脉夹夹闭主动脉弓14 min后再灌注,以建立SCIRI模型; SEVO+I/R组,建立SCIRI模型前吸入2.4%的七氟烷3 h。Basso Beattie Bresnahan评分法评价神经运动功能; TUNEL染色观察细胞凋亡情况; 伊文思蓝(EB)测定血-脊髓屏障(BSCB)通透性; real-time qPCR 检测miR-199a-5p含量; Western blot测定脊髓组织中caspase-9和Bcl-2 水平。结果 与假手术组比较,I/R组神经运动功能评分降低,细胞凋亡率增加,BSCB通透性增加,miR-199a-5p表达降低,caspase-9表达增加,Bcl-2表达减少; 与I/R组比较,SEVO+I/R组神经运动功能评分增加,细胞凋亡率减少,BSCB通透性减少,miR-199a-5p表达增加,caspase-9表达减少,Bcl-2表达增加。结论 七氟烷预处理可能通过增加miR-199a-5p,减少BSCB通透性及细胞凋亡,保护大鼠SCIRI。
Abstract:
Aim To investigate the effect of sevoflurane preconditioning on spinal cord ischemia-reperfusion injury(SCIRI)of miR-199a-5p in rats. Methods Twenty-four SD rats were randomly divided into: group of sham(Sham group), group of ischemia-reperfusion(I/R group), and group of sevoflurane and control(SEVO+I/R group). Sham group received the same operation, but did not inhale sevoflurane or close the aortic arch; SCIRI model was established after 14 min of the aortic arch of the non-invasive arterial clip in I/R group. SEVO+I/R group, before the establishment of SCIRI model, was inhaled 2.4% sevoflurane for 3 h. Basso Beattie Bresnahan scoring method was used to evaluate neuromotor function and TUNEL staining to observe apoptosis. Evans blue(EB)was used to determine blood-spinal cord barrier(BSCB)permeability; real-time qPCR to detect miR-199a-5p content; Western blot to measure the levels of caspase-9 and Bcl-2 in spinal cord tissues. Results Compared with sham group, the score of neuromotor function in I/R group decreased, cell apoptosis rate increased, BSCB permeability increased, miR-199a-5p expression decreased, caspase-9 expression increased, and Bcl-2 expression decreased; compared with I/R group, the neurological motor function score of SEVO+I/R group increased, the apoptotic rate decreased, the BSCB permeability decreased, the expression of miR-199a-5p increased, the expression of caspase-9 decreased, and the expression of Bcl-2 increased. Conclusion Sevoflurane pretreatment may reduce BSCB permeability and neuronal apoptosis by up-regulation of miR-199a-5p and protection of SCIRI in rats.

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备注/Memo

备注/Memo:
收稿日期:2018-05-04,修回日期:2018-06-10
基金项目:国家自然科学基金资助项目(No 81771342)
作者简介: 鲍 宁(1978-), 女, 博士生, 副主任医师, 研究方向:围术期中枢神经系统保护, E-mail:baoning0518@qq.com;
马 虹(1963 -), 女, 博士, 教授, 博士生导师, 研究方向:围术期中枢神经系统保护,通讯作者,E-mail: mahong5466@126.com
更新日期/Last Update: 2018-08-26