[1]李未祥,周大臣,王 石,等.七叶皂苷钠通过CARMA3/NF-κB信号通路抑制肝癌细胞的增殖[J].中国药理学通报,2018,(09):1243-1248.[doi:10.3969/j.issn.1001-1978.2018.09.012]
 LI Wei-xiang,ZHOU Da-chen,WANG Shi,et al.Sodium aescinate inhibits proliferation of hepatocellular carcinoma through CARMA3/NF-κB signaling pathway[J].Chinese Pharmacological Bulletin,2018,(09):1243-1248.[doi:10.3969/j.issn.1001-1978.2018.09.012]
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七叶皂苷钠通过CARMA3/NF-κB信号通路抑制肝癌细胞的增殖()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1243-1248
栏目:
论著
出版日期:
2018-09-26

文章信息/Info

Title:
Sodium aescinate inhibits proliferation of hepatocellular carcinoma through CARMA3/NF-κB signaling pathway
文章编号:
1001-1978(2018)09-1243-06
作者:
李未祥周大臣王 石崔 笑侯 辉耿小平
安徽医科大学第二附属医院肝胆外科,安徽 合肥 230601
Author(s):
LI Wei-xiang ZHOU Da-chen WANG Shi CUI Xiao HOU Hui GENG Xiao-ping
Dept of Hepatobiliary Surgery, the Second Hospital of Anhui Medical University, Hefei 230601, China
关键词:
CARMA3 NF-κB 七叶皂苷钠 肝细胞癌 免疫组化 信号通路
Keywords:
CARMA3 NF-κB sodium aescinate hepatocellular carcinoma immunohistochemistry signaling pathway
分类号:
R284.1; R322.47; R329.24; R329.25; R735.702.2
DOI:
10.3969/j.issn.1001-1978.2018.09.012
文献标志码:
A
摘要:
目的 研究CARMA3、NF-κB在人肝癌组织中的表达及七叶皂苷钠抑制人肝癌细胞增殖的潜在机制。方法 应用组织微阵列免疫组化分析检测100例患者肝癌组织中CARMA3、NF-κB的表达。MTT法测定七叶皂苷钠对肝癌细胞增殖的影响; 流式细胞仪检测细胞凋亡; Western blot和细胞免疫荧光法检测七叶皂苷钠处理后的HepG2和Hep3B细胞株中CARMA3/NF-κB蛋白的表达。结果 100例患者的组织芯片分析显示,与成对的相邻肝组织相比,肝癌组织中CARMA3表达上调,且与临床病理分化、TNM分期、肿瘤体积大小及预后相关。七叶皂苷钠在40 μmol·L-1(IC50)抑制肝癌细胞系的增殖,并能促进其凋亡,但对正常肝细胞无毒性作用。且七叶皂苷钠能够明显抑制CARMA3及其下游NF-κB的表达。结论 七叶皂苷钠通过抑制在肝癌中被激活的CARMA3/NF-κB信号传导通路,从而有效抑制肝癌细胞系的增殖。
Abstract:
Aim To investigate the expression of CARMA3, NF-κB in hepatocellular carcinoma tissues and the underlying mechanism of sodium aescinate in inhibiting the proliferation of human hepatocellular carcinoma cells. Methods The expression of CARMA3 and NF-κB in HCC tissues were detected by tissue microarray immunohistochemistry. MTT was used to determine the effect of sodium aescinate on the proliferation of HCC cells. Cell apoptosis was detected by flow cytometry. The expression of CARMA3,NF-κB protein in HepG2 and Hep3B cells treated with sodium aescinate was detected by Western blot and cell immunofluorescence.Results Tissue microarray analysis showed that the expression of CARMA3 in HCC was up-regulated compared with the adjacent adjacent liver tissues, and the histopathological differentiation, TNM stage, tumor volume and prognosis were correlated. Sodium aescinate in 40 μmol·L-1 concentration(IC50)inhibited the growth of HCC cell lines, promoting its apoptosis, but without toxic effects on normal liver cells. Western blot and cell immunofluorescence detection of sodium aescinate could significantly inhibit the expression of CARMA3 and NF-κB. Conclusion Sodium aescinate can effectively inhibit the proliferation of HCC cells by inhibiting the activation of CARMA3/NF-κB signaling in HCC.

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备注/Memo

备注/Memo:
收稿日期:2018-04-15,修回日期:2018-05-14
基金项目:安徽省高等学校自然科学基金资助项目(No KJ2017A825)
作者简介:李未祥(1989-),男,硕士生,研究方向:肝胆外科,E-mail:lwx19911015@yeah.net;
耿小平(1956-),男,教授,主任医师,博士生导师,研究方向:肝胆胰外科,通讯作者,E-mail:xp_geng@163.com
更新日期/Last Update: 2018-08-26