[1]陆春晓,崔 越,何冬旭,等.大豆皂苷对卵巢癌细胞耐药性的影响[J].中国药理学通报,2018,(09):1249-1253.[doi:10.3969/j.issn.1001-1978.2018.09.13]
 LU Chun-xiao,CUI Yue,HE Dong-xu,et al.Effect of soyasaponin on drug resistance of ovarian cancer cells[J].Chinese Pharmacological Bulletin,2018,(09):1249-1253.[doi:10.3969/j.issn.1001-1978.2018.09.13]
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大豆皂苷对卵巢癌细胞耐药性的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1249-1253
栏目:
论著
出版日期:
2018-08-26

文章信息/Info

Title:
Effect of soyasaponin on drug resistance of ovarian cancer cells
文章编号:
1001-1978(2018)09-1249-05
作者:
陆春晓1崔 越1何冬旭2马 鑫1
江南大学1.药学院和医学院,2.国家重点工程实验室,江苏 无锡 214122
Author(s):
LU Chun-xiao1CUI Yue1HE Dong-xu2MA Xin1
1.School of Pharmaceutical Sciences and Wuxi Medical School; 2.National Engineering Lab for Cereal Fermentation Technology,Jiangnan University,Wuxi,Jiangsu 214122,China
关键词:
大豆皂苷 卵巢癌 耐药 EMT N-cadherin E-cadherin 细胞迁移
Keywords:
soyasaponins ovarian neoplasms drug resistance EMT N-cadherin E-cadherin cell migration
分类号:
R284.1; R329.24; R737.310.22; R915
DOI:
10.3969/j.issn.1001-1978.2018.09.13
文献标志码:
A
摘要:
目的 筛选逆转卵巢癌细胞耐药性效果最佳的大豆皂苷,并研究其可能的作用机制。 方法 用4种常见大豆皂苷分别处理耐紫杉醇卵巢癌细胞A2780/PTX,通过检测细胞IC50值,比较其耐药性变化,从中筛选出逆转耐药性效果最佳的大豆皂苷。应用蛋白免疫印迹和激光共聚焦法检测大豆皂苷处理后,A2780/PTX细胞中上皮-间充质转化(epithelial-mesenchymal transition, EMT)相关标志性蛋白的表达情况; Transwell和划痕实验观察细胞迁移能力的变化。结果 细胞活力检测结果显示,4种常见的大豆皂苷中,Ac逆转A2780/PTX细胞耐药性的效果最明显; 且经大豆皂苷Ac处理48 h后,A2780/PTX细胞中的N-cadherin表达降低,E-cadherin表达升高,细胞的迁移能力降低。结论 大豆皂苷Ac能有效降低A2780/PTX细胞的EMT进程,减弱细胞的迁移能力,逆转其耐药性。
Abstract:
Aim To screen the most effective soyasaponins for reversing the drug resistance of ovarian cancer cells,and investigate the possible mechanism. Methods Cell viability assay was used to analyze the drug resistance of A2780/PTX cells treated with four different common soyasaponins respectively,in order to screen out the most effective soyasaponin. Then,the most effective soyasaponin was used to detect the expression of epithelial-mesenchymal transition(EMT)-related marker proteins,including N-cadherin and E-cadherin,with Western blot and confocal microscopy. Finally,transwell assay and wound healing assay were applied to observe effect of soyasaponin on regulating cancer cell migration. Results Compared with other soyasaponins,soyasaponin Ac most effectively reversed the drug resistance of A2780/PTX cells. The expression of N-cadherin decreased while that of E-cadherin increased in A2780/PTX cells when treated with soyasaponin Ac for 48 h. The results of transwell and wound healing assay suggested that soyasaponin Ac also reduced the migration of A2780/PTX cells. Conclusion Soyasaponin Ac can decrease the drug resistance via EMT pathway and weaken the migration ability of ovarian cancer cells.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-04-14,修回日期:2018-05-22
基金项目:国家自然科学基金资助项目(No 81572940)
作者简介:陆春晓(1993-),女,硕士生,研究方向:分子药理学,E-mail: luchunxiao6@163.com;
马 鑫(1981-),男,博士,教授,博士生导师,研究方向:Trp通道与疾病发生及干预,通讯作者,E-mail: maxin@jiangnan.edu.cn
更新日期/Last Update: 2018-08-26