[1]付 舒,李叶丽,吴雨婷,等.淫羊藿次苷Ⅱ通过调节MMP-2、MMP-9和TIMP-1的表达抗自发性高血压大鼠心肌纤维化[J].中国药理学通报,2018,(09):1253-1257.[doi:10.3969/j.issn.1001-1978.2018.09.014]
 FU Shu,LI Ye-li,WU Yu-ting,et al.Icariside Ⅱ ameliorates myocardial fibrosis in spontaneously hypertensive rats through regulating expression of MMP-2, MMP-9 and TIMP-1[J].Chinese Pharmacological Bulletin,2018,(09):1253-1257.[doi:10.3969/j.issn.1001-1978.2018.09.014]
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淫羊藿次苷Ⅱ通过调节MMP-2、MMP-9和TIMP-1的表达抗自发性高血压大鼠心肌纤维化()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1253-1257
栏目:
论著
出版日期:
2018-09-26

文章信息/Info

Title:
Icariside Ⅱ ameliorates myocardial fibrosis in spontaneously hypertensive rats through regulating expression of MMP-2, MMP-9 and TIMP-1
文章编号:
1001-1978(2018)09-1253-05
作者:
付 舒李叶丽吴雨婷岳 云高 杨杨丹莉
基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室,遵义医学院,贵州 遵义 563003
Author(s):
FU Shu LI Ye-li WU Yu-ting YUE Yun GAO Yang YANG Dan-li
Key Lab of Basic Pharmacology of Ministry of Education and Joint International Research Lab of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi Guizhou 563003,China
关键词:
淫羊藿次苷Ⅱ 心肌纤维化 自发性高血压大鼠 MMP-2 MMP-9 TIMP-1 细胞外基质
Keywords:
icariside Ⅱ myocardial fibrosis spontaneously hypertensive rat MMP-2 MMP-9 TIMP-1 extracellular matrixc
分类号:
R-332; R384.1; R322.11; R345.63; R542.230.22; R544.1; R977.3
DOI:
10.3969/j.issn.1001-1978.2018.09.014
文献标志码:
A
摘要:
目的 观察淫羊藿次苷Ⅱ(icarisid Ⅱ,ICS Ⅱ)对自发性高血压大鼠(spontaneously hypertensive rat, SHR)心肌纤维化的干预作用,并探讨其对基质金属蛋白酶-2(matrix metalloproteinase,MMP-2)、MMP-9及质金属蛋白酶抑制剂(tissue inhibitor of metalloproteinases,TIMPs)的影响。方法 将20只♂SHR大鼠随机分为模型(SHR)组、ICS Ⅱ低、中、高剂量组(ICS Ⅱ-L、ICS Ⅱ-M、ICS Ⅱ-H),♂WKY大鼠为阴性对照(WKY)组。ICS Ⅱ各剂量处理组持续灌胃12周后,检测大鼠收缩血压,处死大鼠分离左心室计算左心质量指数,Masson染色观察心肌胶原含量变化; real time PCR检测左心室组织中MMP-2、MMP-9、TIMP-1 mRNA的表达; Western blot检测左心室组织中MMP-2、MMP-9、TIMP-1、Collagen Ⅰ、Collagen Ⅲ蛋白的表达。结果 ICS Ⅱ(8、16 mg·kg-1)处理组胶原蛋白沉积明显减轻,收缩压和左心质量指数明显下降(P<0.05),左心室组织中MMP-2、MMP-9、Collagen Ⅰ和Collagen Ⅲ表达量明显减少(P<0.05),TIMP-1的表达量明显增加(P<0.05)。结论 ICS Ⅱ可改善SHR大鼠心肌纤维化,其机制可能与降低血压,下调MMP-2、MMP-9,上调TIMP-1的表达有关。
Abstract:
Aim To investigate the effects of Icariside Ⅱ(ICS Ⅱ)on myocardial fibrosis in spontaneously hypertensive rat(SHR). Methods Twenty male SHR rats were randomly divided into the model group(group SHR), ICS Ⅱ low(ICS Ⅱ-L), middle(ICS Ⅱ-M)and high(ICS Ⅱ-H)group, and male WKY rats were set as control group(group WKY). ICS Ⅱ-L, ICS Ⅱ-M and ICS Ⅱ-H groups were intragastrically administered with ICS Ⅱ for 12 weeks. After that the blood pressure was measured in rats. Then, the rats were sacrificed and the left ventricles were separated in order to calculate the left ventricular mass index. Masson staining was used to detect the occurrence of interstitial fibrosis in cardiac tissues. Real time PCR was used to observe the gene expression of MMP-2, MMP-9 and TIMP-1 in the left ventricle in SHRs. The protein expression levels of MMP-2, MMP-9, TIMP-1, Collagen Ⅰ and Collagen Ⅲ were measured by Western blot. Results Compared with SHR group, the myocardial fibrosis was reduced after ICS Ⅱ(8, 16 mg·kg-1)treatment. The blood pressure and left ventricular mass index decreased(P<0.05). The expressions of MMP-2, MMP-9, Collagen Ⅰ and Collagen Ⅲ were down-regulated in left ventricular tissues(P<0.05), while the expression of TIMP-1 was up-regulated(P<0.05). Conclusion Icariside Ⅱ ameliorates myocardial fibrosis in SHR, and the mechanisms might be related to the decrease of blood pressure and down-regulation of MMP-2, MMP-9 expression and up-regulation of TIMP-1 expression.

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备注/Memo

备注/Memo:
收稿日期:2018-05-15,修回日期:2018-06-20
基金项目:国家自然科学基金专项基金(No 81660679)
作者简介:付 舒(1992-),女,硕士生,研究方向:心血管药理学,E-mail:953174009@qq.com;
杨丹莉(1971-),女,博士,教授,硕士生导师,研究方向:心血管药理学,通讯作者,E-mail:zmuyangdanli@foxmail.com
更新日期/Last Update: 2018-08-26