[1]吴永祥,毕淑峰,姜 薇,等.桑白皮多酚对B16细胞内黑色素生成的影响及其机制[J].中国药理学通报,2018,(09):1296-1301.[doi:10.3969/j.issn.1001-1978.2018.09.021]
 WU Yong-xiang,BI Shu-feng,JIANG Wei,et al.Effect of polyphenol from Cortex Mori on melanogenesis of B16 cells and its mechanism[J].Chinese Pharmacological Bulletin,2018,(09):1296-1301.[doi:10.3969/j.issn.1001-1978.2018.09.021]
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桑白皮多酚对B16细胞内黑色素生成的影响及其机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1296-1301
栏目:
论著
出版日期:
2018-08-26

文章信息/Info

Title:
Effect of polyphenol from Cortex Mori on melanogenesis of B16 cells and its mechanism
文章编号:
1001-1978(2018)09-1296-06
作者:
吴永祥12毕淑峰1姜 薇1崔 谱1金有贞2金泰完2
1.黄山学院生命与环境科学学院, 安徽 黄山 245041; 2.安东国立大学食品科学与生物技术学院,韩国 安东 760749
Author(s):
WU Yong-xiang12 BI Shu-feng1 JIANG Wei1 CUI Pu1 KIM You-jeong2 KIM Tae-wan2
1.College of Life and Environment Science, Huangshan University, Huangshan 245041, China; 2.Dept of Food Science and Biotechnology, Andong National University, Andong 760749, Korea
关键词:
桑白皮 多酚 B16细胞 α-黑素细胞刺激素 黑色素 酪氨酸酶
Keywords:
Cortex Mori polyphenol B16 cells α-melanocyte-stimulating hormone melanin tyrosinase
分类号:
R-332; R284.1; R329.24; R345.49; R348.6; R739.5; R977.3; R977.7
DOI:
10.3969/j.issn.1001-1978.2018.09.021
文献标志码:
A
摘要:
目的 研究桑白皮多酚(polyphenol from Cortex Mori,CMP)对小鼠B16细胞内黑色素生成的影响,并探究其作用机制。方法 体外培养小鼠B16细胞,构建α-黑素细胞刺激素(α-MSH)诱导的黑色素高表达细胞模型。CMP干预B16细胞,MTT法测定细胞活性; 分别利用NaOH裂解法和L-Dopa氧化法,分析细胞内黑色素生成含量和酪氨酸酶活性的变化; Western blot和实时荧光定量PCR法分别测定B16细胞中酪氨酸酶(TYR)、酪氨酸酶相关蛋白-1(TRP-1)、酪氨酸酶相关蛋白-2(TRP-2)、小眼畸形相关转录因子(MITF)的蛋白质和mRNA水平。结果 CMP对α-MSH诱导的B16细胞内黑色素生成及酪氨酸酶活力均具有明显的抑制作用(P<0.05),且呈量效关系。当CMP浓度为20 mg·L-1时,对细胞内黑色素生成及酪氨酸酶活性抑制率分别为52.95%、32.85%,阳性对照熊果苷(100 mg·L-1)的抑制率分别为17.29%、16.75%,表明CMP对黑色素生成的抑制效果强于熊果苷。与α-MSH模型组相比,CMP干预后细胞内TYR、TRP-1、TRP-2、MITF的mRNA和蛋白表达被明显抑制(P<0.05)。结论 CMP明显抑制α-MSH诱导黑色素的生成,其机制可能是通过调控TYR、TRP-1、TRP-2、MITF mRNA和蛋白表达,进而抑制酪氨酸酶活性实现的。
Abstract:
Aim To investigate the inhibitory effect of polyphenol from Cortex Mori(CMP)on melanogenesis in mouse melanoma B16 cells and its possible mechanism. Methods Melanoma B16 cells with high expression melanin were induced by α-melanocyte-stimulating hormone(α-MSH)to establish cell model. Cell viability was detected by MTT assay. The melanin synthesis and tyrosinase activity were measured by NaOH and L-Dopa assays, respectively. The tyrosinase(TYR), tyrosinase-related protein-1(TRP-1), tyrosinase-related protein-2(TRP-2)and microphthalmia associated transcription factor(MITF)protein and mRNA levels were measured by Western blot and qRT-PCR, respectively. Results CMP could inhibit the melanin synthesis and tyrosinase activity in α-MSH stimulated B16 cells in a dose-dependent manner(P<0.05). The melanin content and tyrosinase activity significantly decreased by 52.95%, 32.85% at 20 mg·L-1 of CMP, respectively. Treatment of 100 mg·L-1 of arbutin reduced the melanin content and tyrosinase activity by 17.29%, 16.75%, respectively. Based on the results of this study, CMP showed a stronger anti-melanogenesis activity than that of positive control arbutin. After treated by CMP, the protein and mRNA levels of TYR, TRP-1, TRP-2 and MITF were significantly inhibited compared to the α-MSH group(P<0.05). Conclusions CMP could suppress the melanogenesis in α-MSH stimulated B16 cells, and its mechanism may be related to its regulation of the protein and mRNA expressions of TYR, TRP-1, TRP-2 and MITF, and the inhibition of tyrosinase activity.

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备注/Memo

备注/Memo:
收稿日期:2018-05-18,修回日期:2018-06-25
基金项目:安徽省高校自然科学研究重点项目(No KJ2017A398); 安徽省留学回国人员创新项目择优资助计划重点项目(No 2017srst1)
作者简介:吴永祥(1987-),男,博士,讲师,研究方向:天然植物资源功效成分研究及应用,E-mail: wyx2009sun@aliyun.com;
金泰完(1968-),男,博士,教授,研究方向:天然植物资源功效成分研究及应用,通讯作者,E-mail: tk_37@naver.com
更新日期/Last Update: 2018-08-26