[1]常 萍,徐 艳,周大宇,等.银杏多糖对小鼠乳腺癌4T1细胞增殖及GLUT家族基因表达的影响[J].中国药理学通报,2018,(09):1301-1307.[doi:10.3969/j.issn.1001-1978.2018.09.022]
 CHANG Ping,XU Yan,ZHOU Da-yu,et al.Effects of polysaccharides from Ginkgo biloba on proliferation of 4T1 breast cancer cells and expression of GLUT family genes[J].Chinese Pharmacological Bulletin,2018,(09):1301-1307.[doi:10.3969/j.issn.1001-1978.2018.09.022]
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银杏多糖对小鼠乳腺癌4T1细胞增殖及GLUT家族基因表达的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1301-1307
栏目:
论著
出版日期:
2018-09-26

文章信息/Info

Title:
Effects of polysaccharides from Ginkgo biloba on proliferation of 4T1 breast cancer cells and expression of GLUT family genes
文章编号:
1001-1978(2018)09-1301-07
作者:
常 萍1徐 艳1周大宇2吴笳迪2马世良1
沈阳农业大学1.生物科学技术学院、2.食品学院,辽宁 沈阳 110866
Author(s):
CHANG Ping1 XU Yan1 ZHOU Da-yu2 WU Jia-di2 MA Shi-liang1
1.College of Bioscience and Biotechnology, 2.College of Food Science and Technology, Shenyang Agricultural University, Shenyang 110866, China
关键词:
银杏多糖 乳腺癌4T1细胞 增殖 凋亡 葡萄糖转运蛋白家族 葡萄糖转运蛋白1 能量代谢
Keywords:
Ginkgo polysaccharides 4T1 breast cancer cell proliferation apoptosis glucose transporters glucose transporter 1 energy metabolism
分类号:
R-332; R284.1; R329.24; R329.25; R341; R737.9; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.09.022
文献标志码:
A
摘要:
目的 探讨银杏多糖对小鼠乳腺癌4T1细胞增殖、凋亡的影响及可能机制。方法 用不同浓度的银杏多糖处理对数生长期的小鼠乳腺癌4T1细胞,分别用MTT法和台盼蓝拒染法检测银杏多糖对4T1细胞增殖的抑制和细胞毒性作用; 用DAPI核染色检测银杏多糖对4T1细胞凋亡的影响; qRT-PCR检测银杏多糖对4T1细胞葡萄糖转运蛋白家族基因表达的影响。结果 银杏叶和银杏外种皮多糖呈剂量时间依赖性抑制4T1细胞增殖,并且随着银杏多糖剂量的增加,细胞活力明显下降、毒性明显增强,细胞凋亡增加。qRT-PCR结果显示,银杏多糖可以明显降低葡萄糖转运蛋白1的基因表达。结论 银杏多糖可抑制4T1细胞的增殖和诱导细胞凋亡,并通过调节葡萄糖转运蛋白家族基因表达,干预癌细胞的能量代谢,提示银杏多糖对4T1细胞增殖的抑制作用和诱导凋亡作用可能通过干预葡萄糖转运蛋白基因的表达实现的。
Abstract:
Aim To investigate the effects of polysaccharides from Ginkgo biloba on the proliferation, apoptosis of mouse 4T1 breast cancer cells and the possible mechanism. Methods 4T1 cells in logarithmic growth phase were treated with polysaccharides from Ginkgo biloba of different concentrations. The effect of polysaccharides from Ginkgo biloba on inhibition of proliferation and cytotoxicity of 4T1 cells was determined by MTT assay and trypan blue exclusion assay respectively. The apoptotic effect of polysaccharides from Ginkgo biloba on 4T1 cells was detected by DAPI staining. qRT-PCR experiments were carried out for the detection of gene expressions of the glucose transporter family upon the treatment with the polysaccharides from Ginkgo biloba. Results Polysaccharides from either Ginkgo biloba leaf or Ginkgo biloba exocarp significantly inhibited the proliferation of 4T1 cells in a dose- and time-dependent manner. Moreover, with the increasing doses of polysaccharides, cell viability decreased, accompanied by the increased cell cytotoxicity and apoptosis. qRT-PCR results showed that polysaccharides from Ginkgo biloba significantly reduced glucose transporter 1 gene expression. Conclusions Polysaccharides from Ginkgo biloba can both inhibit 4T1 cell proliferation and induce cell apoptosis, and by regulating glucose transporter family gene expression, it interfered with cell energy metabolism, which infers that the effects of cell proliferation inhibition as well the apoptosis induction might be due to the regulation of glucose transporter family gene expression.

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备注/Memo

备注/Memo:
收稿日期:2018-05-18,修回日期:2018-06-20
基金项目:辽宁省十百千高端引进人才项目百人层次项目(No 88030312004)
作者简介:常 萍(1993-),女,硕士生,研究方向:动物细胞与分子生物学,E-mail:18240079146@163.com;
马世良(1961-),男,博士,教授,博士生导师,研究方向:动物细胞与分子生物学,通讯作者,E-mail:msl@syau.edu.cn
更新日期/Last Update: 2018-08-26