[1]陶伟伟,肖 东,吴浩然,等.基于药对探讨中药复方越鞠丸抗抑郁作用配伍规律[J].中国药理学通报,2018,(09):1314-1320.[doi:10.3969/j.issn.1001-1978.2018.09.024]
 TAO Wei-wei,XIAO Dong,WU Hao-ran,et al.The antidepressant effect and mechanism of traditional Chinese formula Yueju Pill based on drug pair[J].Chinese Pharmacological Bulletin,2018,(09):1314-1320.[doi:10.3969/j.issn.1001-1978.2018.09.024]
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基于药对探讨中药复方越鞠丸抗抑郁作用配伍规律()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1314-1320
栏目:
复方药物药理学
出版日期:
2018-09-26

文章信息/Info

Title:
The antidepressant effect and mechanism of traditional Chinese formula Yueju Pill based on drug pair
文章编号:
1001-1978(2018)09-1314-07
作者:
陶伟伟12肖 东1吴浩然1沈佳丽1黄晓燕1薛文达1陈 刚1
南京中医药大学1.中医脑病重点实验室、2.江苏省方剂高技术研究重点实验室, 江苏省中药资源产业化过程协同创新中心,江苏 南京 210023
Author(s):
TAO Wei-wei12 XIAO Dong1 WU Hao-ran1 SHEN Jia-li1 HUANG Xiao-yan1 XUE Wen-da1 CHEN Gang1
1.Key Lab of Integrative Biomedicine of Brain Diseases; 2.Jiangsu Key Lab for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine,Nanjing 210023,China
关键词:
越鞠丸 抗抑郁 栀子-川芎药对 慢性温和不可预见性刺激 免疫印迹法 脑源性神经营养因子
Keywords:
Yueju pil antidepressant effect zhizi and chuanxiong CUMS Western blot BDNF
分类号:
R-332; R289.1; R289.5; R322.81; R749.42; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.09.024
文献标志码:
A
摘要:
目的 以药对研究为切入点,开展中药复方越鞠丸抗抑郁作用配伍规律。方法 在中药复方越鞠丸抗抑郁作用的研究基础上,通过依次拆方获得不同中药药对组合,并通过小鼠急性给药获得越鞠丸中具有抗抑郁作用的关键药对;建立慢性温和不可预知性刺激小鼠模型,进一步验证关键药对的抗抑郁作用,并探讨其分子机制。结果 小鼠急性给药结果表明,栀子与川芎组合为其中发挥抗抑郁作用的关键药对; 在慢性温和不可预知性刺激小鼠模型中,栀子与川芎药对组小鼠糖水消耗明显升高; 悬尾实验及强迫游泳实验的不动时间明显降低; 小鼠海马组织中炎症因子IL-6、TNF-α的水平以及相关炎症蛋白p-NF-κB p65、p-IκBα的表达受到抑制,BDNF、TrkB蛋白表达水平明显上调。结论 栀子-川芎组合为越鞠丸发挥抗抑郁作用的关键药对,该药对可以显著降低栀子产生抗抑郁作用所需剂量,且该作用与其调节小鼠海马组织中炎症因子IL-6、TNF-α与相关炎症蛋白p-NF-κB p65、p-IκBα,以及BDNF及其受体TrkB的表达有关。
Abstract:
Aim To explore the antidepressant mechanism and laws of traditional Chinese formula Yueju Pill by taking drug pair as the breakthrough point. Methods On the basis of anti-depressant activities of Yueju Pill, the combination of different herbs was obtained by the successively disassembling, and the key drug pair was obtained through the acute administration of Yueju Pill in mice. In addition, chronic unpredictable mild stress model was established to further verify the anti-depressant effect of key drug pair, and to explore its molecular mechanism. Results The drug pair of zhizi and chuanxiong was necessary to anti-depression effect of Yueju Pill, and the immobility time of TST and FST was significantly reduced. As expected, the expressions of IL-6 and TNF-a and p-NF-kBp65, P-IkBa were obviously lower than those in model group, but the expressions of BDNF and TrkB were up-regulated than those in model group. Conclusions The drug pair of zhizi and chuanxiong is necessary for traditional Chinese formula Yueju Pill for the antidepressant effect. It is assumed that the antidepressant effect and mechanism of zhizi and chuanxiong are connected with cytokine IL-6, TNF-a and protein expressions of p-NF-kBp65, P-IkBa, and BDNF.

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备注/Memo

备注/Memo:
收稿日期:2018-03-20,修回日期:2018-05-22
基金项目:国家自然科学基金资助项目(No 81673625,81403041); 江苏省自然科学基金资助项目(No BK20151568)
作者简介:陶伟伟(1984-),男,博士,副教授,研究方向:中医药抗抑郁机制,E-mail: tw845@163.com;
陈 刚(1972-),男,博士,教授,博士生导师,研究方向:中医药抗抑郁机制,通讯作者,E-mail:chengang@njucm.edu.cn
更新日期/Last Update: 2018-08-26