[1]陈泽慧,章 亮,柴冰阳,等.一种提高小鼠黑色素瘤在体转移模型成功率的方法[J].中国药理学通报,2018,(09):1326-1330.[doi:10.3969/j.issn.1001-1978.2018.09.026]
 CHEN Ze-hui,ZHANG Liang,CHAI Bing-yang,et al.A method to improve success rate of mice melanoma metastasis model in vivo[J].Chinese Pharmacological Bulletin,2018,(09):1326-1330.[doi:10.3969/j.issn.1001-1978.2018.09.026]
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一种提高小鼠黑色素瘤在体转移模型成功率的方法()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年09期
页码:
1326-1330
栏目:
实验方法学
出版日期:
2018-09-26

文章信息/Info

Title:
A method to improve success rate of mice melanoma metastasis model in vivo
文章编号:
1001-1978(2018)09-1326-05
作者:
陈泽慧章 亮柴冰阳张 波
新疆植物药资源利用教育部重点实验室,石河子大学药学院,新疆 石河子 832002
Author(s):
CHEN Ze-hui ZHANG Liang CHAI Bing-yang ZHANG Bo
Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmaceutics, Shihezi University, Shihezi Xinjiang 832002, China
关键词:
地塞米松 黑色素瘤 肿瘤转移模型 免疫抑制 C57BL/6小鼠 网络药理学
Keywords:
dexamethasone melanoma melanoma metastasis model immunodepression C57BL/6 mice network pharmacology
分类号:
R-332; R322.35; R392.12; R73-37; R734.2; R739.502.2; R979.5
DOI:
10.3969/j.issn.1001-1978.2018.09.026
文献标志码:
A
摘要:
目的 建立一种成功率较高的黑色素瘤转移动物模型,为黑色素瘤转移机制的研究提供可靠的造模方法。 方法 通过网络药理学方法筛选免疫抑制剂; 腹腔注射免疫抑制剂及尾静脉注射B16F10细胞在C57BL/6小鼠建立黑色素瘤转移模型,每天观察小鼠状态,并记录体重; ELISA试剂盒检测血清中TNF-α、IL-6、IL-10、VEGF、MMP-9含量; 解剖小鼠,观察肺部有无转移、转移结节的数目及全身其他器官的转移情况; HE染色确定转移灶组织形态。结果 环孢素及地塞米松的网络接近中心度排名靠前,且靶点较集中于肺和肝脏。造模后小鼠体质量无明显变化,模型组小鼠血清中TNF-α、IL-6、IL-10、VEGF、MMP-9含量增加,肺与肝转移灶数量明显增加,肺组织HE染色显示肿瘤组织呈浸润性生长。结论 通过腹腔注射免疫抑制剂,可以成功增加小鼠黑色素瘤转移模型的成功率。
Abstract:
Aim To improve the rate of melanoma metastasis animal model and provide a reliable experimental modeling method for the study of melanoma metastasis mechanism. Methods Five immunosuppressants were selected and then their targets were screened by network pharmacology. Intraperitoneal injection of immunosuppressants and intravenous injection of B16F10 cells were performed on C57BL/6 mice, then the mice were observed, and body weight were recorded daily. The contents of TNF-α, IL-6, IL-10, VEGF and MMP-9 in serum were measured with ELISA kit. The mice were dissected, then the metastasis situation and the number of metastatic nodules in the lung and other organs were evaluated. HE-staining was involved to determine the morphology of metastatic tissues. Results The closeness centrality of cyclosporine and dexamethasone ranked the top and the targets were concentrated in lung and liver. No significant difference in body weight were observed after animal madel induced. The contents of TNF-α, IL-6 IL-10, VEGF and MMP-9 in the model group increased. The number of metastatic nodules in the lung significantly augmented with some kind of liver metastasis. HE-staining in the lung tissues showed that tumor presented invasive growth. Conclusion By intraperitoneal injection of dexamethasone, the success rate of mice melanoma metastasis model can be greatly increased.

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备注/Memo

备注/Memo:
收稿日期:2018-03-23,修回日期:2018-06-18
基金项目:国家自然科学基金资助项目(No 81460566,U1603122)
作者简介:陈泽慧(1990 -),女,硕士生,研究方向:系统药理学与肿瘤药理学,E-mail:252090932@qq.com;
张 波(1978-),男,博士,教授,研究方向:系统药理学与中药药理学,通讯作者,E-mail:bozhang_lzu@126.com
更新日期/Last Update: 2018-08-26