[1]戴临风,谢兆玉,金佳琪,等.基于ROS/TXNIP/Nlrp3探讨芦荟大黄素对P.g-LPS诱导的小鼠牙周膜成纤维细胞的影响[J].中国药理学通报,2018,(10):1368-1373.[doi:10.3969/j.issn.1001-1978.2018.10.009]
 DAI Lin-feng,XIE Zhao-yu,JIN Jia-qi,et al.Effect of aloe-emodin on mouse periodontal ligament fibroblasts by P.g-LPS stimulated based on ROS/TXNIP/NLRP3 signaling pathway[J].Chinese Pharmacological Bulletin,2018,(10):1368-1373.[doi:10.3969/j.issn.1001-1978.2018.10.009]
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基于ROS/TXNIP/Nlrp3探讨芦荟大黄素对P.g-LPS诱导的小鼠牙周膜成纤维细胞的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年10期
页码:
1368-1373
栏目:
论著
出版日期:
2018-09-26

文章信息/Info

Title:
Effect of aloe-emodin on mouse periodontal ligament fibroblasts by P.g-LPS stimulated based on ROS/TXNIP/NLRP3 signaling pathway
文章编号:
1001-1978(2018)10-1368-06
作者:
戴临风1谢兆玉1金佳琪1赖洁青2连大卫1黄琳砚4黄 怡34陈 扬1
1.广州中医药大学 中药学院,广东 广州 510006; 2.广州中医药大学 第一临床医学院,广东 广州 510006; 3.暨南大学附属第一医院口腔医疗中心,广东 广州 510630; 4.暨南大学口腔医学院, 广东 广州 510630
Author(s):
DAI Lin-feng1 XIE Zhao-yu1 JIN Jia-qi1 LAI Jie-qing2 LIAN Da-wei1 HUANG Lin-yan4 HUANG Yi34 CHEN Yang1
1.School of Pharmaceutical,2.the First School of Clinical Medicine, Guangzhou University of Chinese Medicine,Guangzhou 510000, China; 3.Dept of Stomatology, the First Affiliated Hospital, 4.School of Dental Medicine, Jinan University, Guangzhou 510630, China
关键词:
芦荟大黄素 Nlrp3-inflammasome ROS TXNIP 牙周膜成纤维细胞 caspase-1
Keywords:
aloe-emodin NLRP3 inflammasome ROS TXNIP mouse periodontal ligament fibroblasts caspase-1
分类号:
R-332; R284.1; R329.24; R364.5; R781.402.2; R977.6
DOI:
10.3969/j.issn.1001-1978.2018.10.009
文献标志码:
A
摘要:
目的 研究芦荟大黄素(aloe-emodin, AE)对牙龈卟啉单胞菌脂多糖(P.g-LPS)诱导的牙周膜成纤维细胞Nlrp3-inflammasome活化的影响。方法 体外培养牙周膜成纤维细胞,采用P.g-LPS作为刺激剂,通过Western blot观察不同浓度芦荟大黄素(aloe-emodin, AE)对Nlrp3、前体caspase-1(pro-caspase-1)、活化caspase-1(cle-caspase-1)、TXNIP蛋白表达量的影响分子活化程度,利用RT-qPCR方法检测其对前体caspase-1、Nlrp3转录水平的影响,并使用荧光探针法评价对ROS生成量的影响。结果 与空白对照相比,给予P.g-LPS刺激的小鼠牙周膜成纤维细胞Nlrp3、前体caspase-1、TXNIP的蛋白表达量及ROS的生成量明显增高,活化caspase-1的生成明显增多,AE可以明显降低Nlrp3、前体caspase-1,活化caspase-1,TXNIP在细胞内的蛋白表达量,同时抑制Nlrp3和前体caspase-1的转录水平并且减少ROS在细胞内的生成量。结论 AE能通过抑制Nlrp3相关蛋白的表达及减弱ROS/TXNIP诱导的Nlrp3-inflammasome聚集明显抑制P.g-LPS诱导的小鼠牙周膜成纤维细胞Nlrp3-inflammasome的活化。
Abstract:
Aim To investigate the protective effect of aloe-emodin(AE)on mouse periodontal ligament fibroblasts(mPDLFs)induced by P.g-LPS of Porphyromonas gingivalis and the relevant mechanism.Methods P.g-LPS was used as the stimuli to induce mPDLFs and Western blotting was employed to investigate the effect of AE on the expression of NLRP3, Pro-caspase-1, Cle-caspase-1 and TXNIP in P.g-LPS-treated mPDLFs.The effects of AE on the transcription levels of Pro-caspase-1 and NLRP3 were further detected by RT-qPCR.In addition, chemical fluorescence was used to investigate the impact of AE on production of ROS in mPDLFs treated by P.g-LPS.Results Compared with control group, P.g-LPS increased the expression of NLRP3, Pro-caspase-1, Cle-caspase-1, TXNIP and the transcription levels of NLRP3 and Pro-caspase-1 and the production of ROS in mPDLFs.AE could decrease the expression of NLRP3, Pro-caspase-1 and Cle-caspase-1 in varying degrees and inhibited the transcription of NLRP3 and Pro-caspase-1.Meanwhile, the production of ROS and the expression of TXNIP were also reduced by AE.Conclusions AE may inhibit the activation of NLRP3 inflammasome in P.g-LPS treated mPDLFs by reducing NLRP3 related protein formation and NLRP3 inflammasome gathering through ROS/TXNIP.

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备注/Memo

备注/Memo:
收稿日期:2018-06-28,修回日期:2018-07-28
基金项目:国家自然科学基金资助项目(No 81603587,No 81603668),国家重点研发计划(No 2017YFC1700400),广东省杰出青年科学基金(No 2018B030306027),广东省科技计划项目(No 2017A020211016),中华中医药学会青托人才项目(No CACM-2017-QNRC2-C12),中央高校基本科研业务费专项资金(No11616334),广东省中医药局(No 20161076),全国大学生创新创业训练项目(No 201710572165),广东大学生科技创新培育专项资金项目(批准号:pdjh2017a0114)
作者简介:戴临风(1997-),男,本科,研究方向:中药药理,E-mail:1435819402@qq.com
通讯作者:陈 扬(1983-),男,教授,博士生导师,研究方向:中药药理,Tel:(020)39357021,E-mail:ychen8@gzucm.edu.cn
更新日期/Last Update: 2018-08-26