[1]顾子杨,崔梦迪,李玉华,等.黑枸杞花色苷纯化工艺的优化与评价[J].中国药理学通报,2018,(10):1373-1379.[doi:10.3969/j.issn.1001-1978.2018.10.010]
 GU Zi-yang,CUI Meng-di,LI Yu-hua,et al.Optimization and evaluation of purification process of anthocyanins in Lyceum ruthenicum Murr.[J].Chinese Pharmacological Bulletin,2018,(10):1373-1379.[doi:10.3969/j.issn.1001-1978.2018.10.010]
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黑枸杞花色苷纯化工艺的优化与评价()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年10期
页码:
1373-1379
栏目:
论著
出版日期:
2018-10-26

文章信息/Info

Title:
Optimization and evaluation of purification process of anthocyanins in Lyceum ruthenicum Murr.
文章编号:
1001-1978(2018)10-1373-07
作者:
顾子杨1崔梦迪2李玉华1季 拓1潘 扬1潘自皓1
1.南京中医药大学药学院,江苏 南京 210023; 2.南京中医药大学第三附属医院甲乳外科,江苏 南京 210001
Author(s):
GU Zi-yang1CUI Meng-di2LI Yu-hua1JI Tuo1PAN Yang1PAN Zi-hao1
1.Pharmacy School,Nanjing University of Chinese Medicine,Nanjing 210023,China; 2.Thyroid and Breast Surgery,No.3 Affiliated Hospital,Nanjing University of Chinese Medicine,Nanjing 210001,China
关键词:
黑枸杞 花色苷 纯化 优化 大孔吸附树脂 抗氧化活性
Keywords:
Lyceum ruthenicum Murr. anthocyanin purification optimization macroporous adsorption resin antioxidant activity
分类号:
R282.71; R284.1; R284.2
DOI:
10.3969/j.issn.1001-1978.2018.10.010
文献标志码:
A
摘要:
目的 优化黑枸杞花色苷提取物的纯化工艺,并评价最优方法纯化效果。方法 先后考察了黑枸杞花色苷的萃取工艺和大孔吸附树脂分离工艺,并采用高效液相色谱分析和自由基清除率测定,分别评价提取、萃取与大孔吸附树脂分离产物的纯度和抗氧化活性。结果 先采用水饱和过夜3倍体积的乙酸乙酯萃取提取液4次,每次12 h; 再采用药材浓度为200 g·L-1的萃取液,以1 mL·min-1的流速上样大孔吸附树脂柱,在洗涤10个BV后,使用70%乙醇,以1 mL·min-1的流速洗脱树脂柱,获得的纯化产物组成完整,纯度明显提高,抗氧化活性也明显增加。结论 初步建立了效果理想的黑枸杞花色苷纯化工艺。
Abstract:
Aim To optimize and evaluate the purification process of anthocyanins in Lyceum ruthenicum Murr.(LRM).Methods The solvent extraction technology and separation technology using macroporous adsorption resin of anthocyanins in LRM was investigated,and the purities and the antioxidant activities of its crude extraction,solvent extraction and macroporus resin separation products were assessed by high performance liquid chromatography(HPLC)analysis and free radical scavenging assay respectively.Results The optimized purification conditions were as follows:the crude extract of anthocyanins in LRM was liquid-liquid extracted four times by water-saturated ethyl acetate at a ratio of 1:3 for 12 h each time,and following loaded into macroporous adsorption resin at a flow rate of 1 mL·min-1; the sample at crude drug concentration of 200 g·L-1 was rinsed with 1% formic acid for 10 bed volumes and then eluted by 70% ethanol until the effluent was colorless at a flow rate of 1 mL·min-1.After purified under above purification conditions,the composition of the product was complete,and the purity and the antioxidant activity of the product was significantly enhanced.Conclusion The purification process of anthocyanins in LRM iswas successfully established.

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备注/Memo

备注/Memo:
收稿日期:2018-05-16,修回日期:2018-07-24
基金项目:江苏高校优势学科建设工程项目(PAPD); 江苏高校品牌专业建设工程资助项目(No PPZY2015A070); 南京中医药大学校企合作项目(No 2015001)
作者简介:顾子杨(1991-),男,硕士生,研究方向:生物制药,E-mail:guziyang1113@163.com;
潘 扬(1964-),男,博士,教授,研究方向:生物制药,通讯作者,E-mail:y.pan2006@163.com;
潘自皓(1981-),男,博士,助理研究员,研究方向:生物制药,通讯作者,E-mail:Lance814@163.com
更新日期/Last Update: 2018-08-26