[1]柯 旋,李宾公,郝艳琴,等.Vaspin在糖尿病心肌病中的作用及机制研究[J].中国药理学通报,2018,(10):1403-1408.[doi:10.3969/j.issn.1001-1978.2018.10.015]
 KE Xuan,LI Bin-gong,HAO Yan-qin,et al.Role and mechanism of vaspin in diabetic cardiomyopathy[J].Chinese Pharmacological Bulletin,2018,(10):1403-1408.[doi:10.3969/j.issn.1001-1978.2018.10.015]
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Vaspin在糖尿病心肌病中的作用及机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年10期
页码:
1403-1408
栏目:
论著
出版日期:
2018-09-26

文章信息/Info

Title:
Role and mechanism of vaspin in diabetic cardiomyopathy
文章编号:
1001-1978(2018)10-1403-06
作者:
柯 旋12李宾公1郝艳琴1李雪莲1邹 晋3
1.南昌大学第一附属医院心内科,江西 南昌 330006; 2.武汉市第三医院心内科,湖北 武汉 430000; 3.江西省人民医院心内科,江西 南昌 330006
Author(s):
KE Xuan12 LI Bin-gong1 HAO Yan-qin1 LI Xue-lian1 ZOU Jin3
1.Dept of Cardiology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China; 2.Dept of Cardiology, the Third Hospital of Wuhan, Wuhan 430000, China; 3.Dept of Cardiology, Provincial People's Hospital, Nanchang 330006, China
关键词:
vaspin 自噬 心肌纤维化 糖尿病心肌病 凋亡 氧化应激
Keywords:
vaspin autophagy fibrosis DCM apoptosis oxidative stress
分类号:
R-332; R322.11; R329.25; R331.31; R542.23; R587.202.2; R977.3
DOI:
10.3969/j.issn.1001-1978.2018.10.015
文献标志码:
A
摘要:
目的 观察内脏脂肪丝氨酸蛋白酶抑制剂vaspin对糖尿病心肌病(diabetic cardiomyopathy, DCM)大鼠心肌纤维化及心功能的影响。方法 ♂SD大鼠一次性腹腔注射链脲霉素(60 mg·kg-1)制备糖尿病模型,模型成功后12周为DCM组,对照组腹腔注射生理盐水(0.5 mL·kg-1·d-1),vaspin组为模型成功后腹腔注射vaspin(320 ng·kg-1·d-1)。心脏彩超观察大鼠左室射血分数(LVEF)、短轴收缩率(FS)、左室舒张末内径(LVEDd)、左室收缩末内径(LVEDs),并检测E峰/A峰(Em/Am); ELISA检测大鼠血清中TNF-α水平; Masson染色检测心肌胶原纤维,并分析胶原容积分数(CVF); Western blot测定心肌组织中自噬及凋亡蛋白表达。结果 与对照组相比,DCM组TNF-α升高(P<0.01),LVEF、FS、Em/Am降低(P<0.01),LC3-II/LC3-I、Beclin-1表达降低(P<0.01),Collagen I、TGF-β1、p62、Bax/Bcl-2升高(P<0.01); 与DCM组比较,vaspin组LVEF、FS、Em/Am升高(P<0.01),LC3-II/LC3-I、Beclin-1升高(P<0.01),Collagen I、p62、Bax/Bcl-2降低(P<0.01),TNF-α和TGF-β1无明显变化(P>0.05)。结论 Vaspin可增加DCM大鼠心肌组织自噬水平, 改善DCM大鼠心肌纤维化。
Abstract:
Aim To observe the effects of vaspin on myocardial fibrosis and cardiac function in diabetic cardiomyopathy(DCM)rats.Methods Male SD rats were divided randomly into three groups: control group, DCM group, and vaspin group.Rats in control group were simply treated with normal saline(0.5 mL·kg-1·d-1)and in vaspin group were injected intraperitoneally with vaspin(320 ng·kg-1·d-1)solution in normal saline daily for 12 weeks after diabetes was developed.Then the following experiments were performed: Echocardiography to assess left ventricular ejection fraction(LVEF), fractional shortening(FS), left ventricular end-diastole diameter(LVIDd), left ventricular end-systolic diameter(LVIDs)and Em/Am; ELISA to measure serum TNF-α concentratio; Masson's trichrome to assess cardiac fibrosis and analyse collagen volume fraction(CVF); Western blot to examine the expressions of LC3, Beclin-1, P62, Bax, Bcl-2, TGF-β1 and collagen I in the myocardium.Results As for TNF-α levels, there was no statistically significant difference between DCM group and vaspin group, statistically significant difference between DCM and control group was revealed.Echocardiography performed to assess the effect of vaspin on cardiac function showed that LVEF and FS levels decreased in DCM group while increased in vaspin group; the ratio of Em/Am decreased in DCM group but was not improved in vaspin group.Western blot showed that LC3-II/LC3-I ratio and Beclin-1 expression decreased and P62 expression increased in DCM group, which were reversed by vaspin treatment, however.Compared with DCM group, collagen deposition in vaspin group decreased.Western blot suggested that vaspin could reduce the expression of collagen I, but had no significant effect on the expression of TGF-β1.Conclusions Vaspin increases the level of autophagy and could ameliorate myocardial fibrosis and cardiac function in rats with diabetic cardiomyopathy.

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备注/Memo

备注/Memo:
收稿日期:2018-06-10,修回日期:2018-07-12
基金项目:国家自然科学基金资助项目(No 81460046); 江西省教育厅重点科研项目(No GJJ160022)
作者简介:柯 旋(1991-),男,硕士生,研究方向:脂肪因子、糖尿病心肌病心肌纤维化机制,E-mail: kexuan1027@163.com;
李宾公(1972 -),男,博士,教授,博士生导师,研究方向:心肌纤维化机制,通讯作者,E-mail: libingong08@163.com
更新日期/Last Update: 2018-08-26