[1]沈 芹,柳桂萍,王雯雯,等.金雀异黄素对血小板微颗粒诱导的类风湿关节炎成纤维样滑膜细胞迁移和侵袭的影响[J].中国药理学通报,2018,(10):1408-1413.[doi:10.3969/j.issn.1001-1978.2018.10.016]
 SHEN Qin,LIU Gui-ping,WANG Wen-wen,et al.Effects of genistein on migration and invasion of fibroblast-like synoviocytes from rheumatoid arthritis induced by platelet-derived microparticles[J].Chinese Pharmacological Bulletin,2018,(10):1408-1413.[doi:10.3969/j.issn.1001-1978.2018.10.016]
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金雀异黄素对血小板微颗粒诱导的类风湿关节炎成纤维样滑膜细胞迁移和侵袭的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年10期
页码:
1408-1413
栏目:
论著
出版日期:
2018-09-26

文章信息/Info

Title:
Effects of genistein on migration and invasion of fibroblast-like synoviocytes from rheumatoid arthritis induced by platelet-derived microparticles
文章编号:
1001-1978(2018)10-1408-06
作者:
沈 芹1柳桂萍2王雯雯1胡 君3周 玮4刘家欢1王 慧1沈维干5张 育15
1.扬州大学附属苏北人民医院风湿免疫科,江苏 扬州 225001; 2.泰州市人民医院风湿免疫科,江苏 泰州 225300; 3.江苏省武警总队医院内科,江苏 扬州 225001; 4.扬州大学附属医院血液风湿科,江苏 扬州 225001; 5.扬州大学医学院,江苏 扬州 225001
Author(s):
SHEN Qin1 LIU Gui-ping2 WANG Wen-wen1 HU Jun3 ZHOU Wei4 LIU Jia-huan1 WANG Hui1 SHEN Wei-gan5 ZHANG Yu15
1.Dept of Rheumatology and Immunology, North Jiangsu People's Hospital of Jiangsu Province Affiliated to Yangzhou University, Yangzhou, Jiangsu 225001, China; 2.Dept of Rheumatology and Immunology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, China; ...
关键词:
类风湿关节炎 金雀异黄素 成纤维样滑膜细胞 细胞迁移 细胞侵袭 NF-κB
Keywords:
rheumatoid arthritis genistein fibroblast-like synoviocyte cell migration cell invasion NF-κB
分类号:
R284.1; R322.72; R329.24; R593.22
DOI:
10.3969/j.issn.1001-1978.2018.10.016
文献标志码:
A
摘要:
目的 探讨金雀异黄素(genistein,Gen)对血小板微颗粒(platelet-derived microparticles,PMPs)诱导的类风湿关节炎(rheumatoid arthritis,RA)成纤维样滑膜细胞(fibroblast-like synoviocyte,FLS)迁移和侵袭的影响,并探究其可能的机制。方法 应用CCK-8试剂盒检测Gen对PMPs作用后RA-FLS活力的影响; 通过Transwell细胞迁移、侵袭实验,以及细胞与细胞外基质(ECM)的黏附实验,探究Gen对PMPs诱导的RA-FLS迁移、侵袭以及与ECM黏附的影响; 免疫荧光染色观察Gen对PMPs作用后RA-FLS应力纤维的分布、丝状和片状伪足形成的影响; Western blot检测Gen对PMPs作用后RA-FLS中NF-κB信号通路组分的影响。结果 Gen对PMPs诱导的RA-FLS的活力无明显影响; 然而,Gen可抑制PMPs诱导的RA-FLS的迁移、侵袭及黏附; 同时,Gen可减少PMPs作用后RA-FLS片状伪足的形成,促进应力纤维的形成。此外,Gen能下调PMPs诱导的RA-FLS中p-IκB、p-NF-κB的表达。结论 Gen可抑制PMPs激活的NF-κB信号通路,影响RA-FLS的肌动蛋白细胞骨架装配,从而抑制PMPs诱导的RA-FLS与ECM的黏附、迁移和侵袭。
Abstract:
Aim To explore the effects of genistein(Gen)on the migration and invasion of fibroblast-like synoviocyte(FLS)from rheumatoid arthritis(RA)induced by platelet-derived microparticles(PMPs), and to reveal the underlying mechanism.Methods The CCK-8 kits were performed to examine the effects of Gen on cell viability of RA-FLS induced by PMPs.Transwell cell migration, invasion and cell-matrix adhesion assays were used to examine the effects of Gen on the migration, invasion and adhesion onto the extracellular matrix(ECM)of RA-FLS stimulated by PMPs.The immunofluorescence assay was applied to observe the effects of Gen on the distribution of stress fibers and the formation of filopodia and lamellipodia in RA-FLS after treatment with PMPs.Western blotting was performed to detect the effects of Gen on the expression of basic components of NF-κB signaling pathway in RA-FLS after treatment with PMPs.Results The effects of Gen on viability of RA-FLS induced by PMPs showed no statistical differences, whereas Gen could suppress the migration, invasion and adhesion to ECM, and decrease the lamellipodia formation and increase the stress fibre formation in RA-FLS induced by PMPs.Moreover, Gen down-regulated the levels of p-IκB and p-NF-κB in RA-FLS incubated by PMPs.Conclusions Gen can inhibit cell adhesion onto ECM, migration and invasion of RA-FLS induced by PMPs presumably via regulating NF-κB signaling pathways activated by PMPs, and thereby modulating the F-actin cytoskeleton rearrangement of RA-FLS.

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备注/Memo

备注/Memo:
收稿日期:2018-05-15,修回日期:2018-06-18
基金项目:国家自然科学基金资助项目(No 81470070,81771728)
作者简介:沈 芹(1990-),女,硕士生,研究方向:类风湿关节炎的基础与临床,E-mail:1169450638@qq.com;
张 育(1960-),男,硕士,教授,硕士生导师,研究方向:炎性关节病的基础与临床,通讯作者,E-mail:yzzy10182001@aliyun.com
更新日期/Last Update: 2018-08-26