[1]韩 瑞,张 配,王尚华,等.下调microRNA-17增强奥沙利铂对口腔癌细胞诱导凋亡作用[J].中国药理学通报,2018,(10):1434-1439.[doi:10.3969/j.issn.1001-1978.2018.10.021]
 HAN Rui,ZHANG Pei,WANG Shang-hua,et al.Down-regulation of microRNA-17 enhances effect of oxaliplatin on induced apoptosis of oral cancer cells[J].Chinese Pharmacological Bulletin,2018,(10):1434-1439.[doi:10.3969/j.issn.1001-1978.2018.10.021]
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下调microRNA-17增强奥沙利铂对口腔癌细胞诱导凋亡作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年10期
页码:
1434-1439
栏目:
论著
出版日期:
2018-10-26

文章信息/Info

Title:
Down-regulation of microRNA-17 enhances effect of oxaliplatin on induced apoptosis of oral cancer cells
文章编号:
1001-1978(2018)10-1434-06
作者:
韩 瑞1张 配2王尚华1程如玉1刘 浩2徐锦程1
蚌埠医学院1.第一附属医院口腔科、2.药学院,安徽 蚌埠 233030
Author(s):
HAN Rui1ZHANG Pei2WANG Shang-hua1CHENG Ru-yu1LIU Hao2XU Jin-cheng1
1.Dept of Stomatology,the First Affiliated Hospital of Bengbu Medical College, 2.School of Pharmacy,Bengbu Medical College,Bengbu Anhui 233030,China
关键词:
microRNA-17 奥沙利铂 口腔癌 舌癌 凋亡 药物敏感
Keywords:
microRNA-17 oxaliplatin oral cancer tongue cancer apoptosis chemosensitivity
分类号:
R329.25; R342.2; R739.8; R979.1
DOI:
10.3969/j.issn.1001-1978.2018.10.021
文献标志码:
A
摘要:
目的 探究下调microRNA-17增强奥沙利铂(oxaliplatin,OXA)对人口腔肿瘤细胞增殖及凋亡的作用及其机制。方法 使用不同浓度的OXA处理人舌鳞状细胞癌TCA8113、人口腔癌KB细胞,MTT法检测转染microRNA-17抑制剂前后对细胞增殖的影响; 采用线粒体膜电位检测试剂盒,检测细胞内线粒体膜电位的变化; DAPI染色,倒置荧光显微镜观察细胞核凋亡情况; Western blot检测Bax、Bcl-2、Mcl-1表达。结果 单独使用OXA 或转染microRNA-17抑制剂均可抑制TCA8113、KB细胞的增殖,使用2 μmol·L-1 OXA作用于TCA8113、KB细胞24 h后,细胞存活率为69.99%、60.77%; 下调microRNA-17后,2 μmol·L-1 OXA作用于口腔癌细胞24 h后,细胞存活率为51.26%、39.27%。JC-1荧光检测发现,红色荧光减弱,绿色荧光增强,提示线粒体膜电位下降; DAPI染色后观察到,相对于单独应用奥沙利铂组,下调microRNA-17与奥沙利铂联合作用组细胞核碎裂像增多; 下调microRNA-17可增强奥沙利铂对口腔癌细胞促凋亡蛋白Bax、Mcl-1表达,抑制细胞Bcl-2的表达。结论 下调microRNA-17可增强奥沙利铂对口腔癌细胞诱导凋亡作用,为口腔癌的治疗提供新思路。
Abstract:
Aim To investigate the down-regulation of microRNA-17 enchancing the effect of oxaliplatin(OXA)on induced apoptosis of human tongue squamous cells and the underlying mechanism.Methods After being treated with OXA and transfected with microRNA-17 inhibitor alone or in combination,the cell proliferation of TCA8113 and KB cells was measured by MTT.The mitochondrial membrane potential was measured by JC-1 assay.The cell nuclear morphology was observed after DAPI staining.The expressions of Bax,Bcl-2,and Mcl-1 in cells were measured with Western blot.Results Both OXA treatment and microRNA-17 inhibitor transfection inhibited the proliferation of TCA8113 and KB cells.After being treated with OXA(2 μmol·L-1)in TCA8113 and KB cells for 24 h,the viability of cells was 69.99% and 60.77%,respectively,while for the treatment of OXA(2 μmol·L-1 )combined with miR-17 inhibitor,the viability of cells was 51.26%,39.27%,respectively,indicating a stronger inhibitory effect on the proliferation of TCA8113 and KB cells than OXA treatment alone.JC-1 fluorescence detection revealed red fluorescence attenuation and green fluorescence enhancement,suggesting decreased mitochondrial membrane potential.After DAPI staining,it was observed that the nuclear fragmentation increased compared to the isolated application of oxaliplatin group.The expression of Bcl-2 in cells decreased,while that of a Bax and Mcl-1 increased in cells.Conclusion The down-regulation of microRNA-17 enhances the effect of oxaliplatin on induced apoptosis of oral cancer cells,suggesting the potential of microRNA-17 as a new candidate target for clinical treatment of oral squamous carcinoma.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-06-15,修回日期:2018-07-18
基金项目:安徽省高校自然科学研究重点项目(No KJ2016A480)
作者简介:韩 瑞(1992-),男,硕士生,研究方向:口腔颌面部肿瘤,E-mail:chinahan521@qq.com;
徐锦程(1965-),男,硕士,教授,硕士生导师,研究方向:口腔颌面部肿瘤,通讯作者,E-mail:xjch9999@163.com;
刘 浩(1979-),男,博士,教授,硕士生导师,研究方向:生化药理学,通讯作者,E-mail:liuhao6886@foxmail.com
更新日期/Last Update: 2018-08-26